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1bos

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SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR

Structural highlights

1bos is a 20 chain structure with sequence from Enterobacteria phage H-19B and Phage h30. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

STXB_BPH19 The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.

Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3.,Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ. Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3. Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303 doi:10.1021/bi971806n

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bos"

@@ Line 1: / Line 1: @@
 ==SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR==
-<StructureSection load='1bos' size='340' side='right' caption='[[1bos]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='1bos' size='340' side='right'caption='[[1bos]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Bph30 Bph30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BOS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_H-19B Enterobacteria phage H-19B] and [https://en.wikipedia.org/wiki/Phage_h30 Phage h30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [http://pdbe.org/1bos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [http://www.ebi.ac.uk/pdbsum/1bos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [https://pdbe.org/1bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [https://www.ebi.ac.uk/pdbsum/1bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/STXB_BPH30 STXB_BPH30]] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
+[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[Shiga toxin|Shiga toxin]]
+*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bph30]]
+[[Category: Enterobacteria phage H-19B]]
-[[Category: Armstrong, G D]]
+[[Category: Large Structures]]
-[[Category: Boodhoo, A]]
+[[Category: Phage h30]]
-[[Category: Brunton, J L]]
+[[Category: Armstrong GD]]
-[[Category: Cummings, M D]]
+[[Category: Boodhoo A]]
-[[Category: Hazes, B]]
+[[Category: Brunton JL]]
-[[Category: Ling, H]]
+[[Category: Cummings MD]]
-[[Category: Read, R J]]
+[[Category: Hazes B]]
-[[Category: Ob-fold]]
+[[Category: Ling H]]
-[[Category: Protein-carbohydrate recognition]]
+[[Category: Read RJ]]
-[[Category: Receptor binding]]
-[[Category: Toxin]]

1bos

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Current revision

SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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