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| | <StructureSection load='1bxm' size='340' side='right'caption='[[1bxm]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='1bxm' size='340' side='right'caption='[[1bxm]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1bxm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halophytophthora_cryptogea Halophytophthora cryptogea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1BXM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1bxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_cryptogea Phytophthora cryptogea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BXM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERG:ERGOSTEROL'>ERG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CRY (MUTATED LYS 13 HIS) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4786 Halophytophthora cryptogea])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERG:ERGOSTEROL'>ERG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1bxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bxm OCA], [http://pdbe.org/1bxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bxm RCSB], [http://www.ebi.ac.uk/pdbsum/1bxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bxm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bxm OCA], [https://pdbe.org/1bxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bxm RCSB], [https://www.ebi.ac.uk/pdbsum/1bxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bxm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ELIB_PHYCR ELIB_PHYCR]] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. | + | [https://www.uniprot.org/uniprot/ELIB_PHYCR ELIB_PHYCR] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Halophytophthora cryptogea]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boissy, G]]
| + | [[Category: Phytophthora cryptogea]] |
| - | [[Category: Brunie, S]]
| + | [[Category: Boissy G]] |
| - | [[Category: Elicitin]]
| + | [[Category: Brunie S]] |
| - | [[Category: Fungal toxic elicitor]]
| + | |
| - | [[Category: Fungal toxic elicitor mutant]]
| + | |
| - | [[Category: Phytophthora]] | + | |
| - | [[Category: Plant pathogen]] | + | |
| - | [[Category: Sterol]] | + | |
| Structural highlights
Function
ELIB_PHYCR Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Elicitins, produced by most of the phytopathogenic fungi of the genus Phytophthora, provoke in tobacco both remote leaf necrosis and the induction of a resistance against subsequent attack by various microorganisms. Despite the recent description of the three-dimensional crystal structure of cryptogein (CRY), the molecular basis of the interactions between Phytophthora and plants largely remains unknown. The X-ray crystal structure, refined at 2.1 A, of a ligand complexed, mutated CRY, K13H, is reported. Analysis of this structure reveals that CRY is able to encapsulate a ligand that induces only a minor conformational change in the protein structure. The ligand has been identified as an ergosterol by gas chromatographic analysis coupled with mass spectrometry analysis. This result is consistent with biochemical data that have shown that elicitins are a distinct class of Sterol Carrier Proteins (SCP). Data presented here provide the first structural description of the pertinent features of the elicitin sterol interaction and permit a reassessment of the importance of both the key residue 13 and the mobility of the omega loop for the accessibility of the sterol to the cavity. The biological implications thereof are discussed. This paper reports the first structure of a SCP/sterol complex.
The 2.1 A structure of an elicitin-ergosterol complex: a recent addition to the Sterol Carrier Protein family.,Boissy G, O'Donohue M, Gaudemer O, Perez V, Pernollet JC, Brunie S Protein Sci. 1999 Jun;8(6):1191-9. PMID:10386869[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Boissy G, O'Donohue M, Gaudemer O, Perez V, Pernollet JC, Brunie S. The 2.1 A structure of an elicitin-ergosterol complex: a recent addition to the Sterol Carrier Protein family. Protein Sci. 1999 Jun;8(6):1191-9. PMID:10386869
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