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| | <StructureSection load='1c8c' size='340' side='right'caption='[[1c8c]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='1c8c' size='340' side='right'caption='[[1c8c]], [[Resolution|resolution]] 1.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1c8c]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C8C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1c8c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8C FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8c OCA], [http://pdbe.org/1c8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c8c RCSB], [http://www.ebi.ac.uk/pdbsum/1c8c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8c ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8c OCA], [https://pdbe.org/1c8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8c RCSB], [https://www.ebi.ac.uk/pdbsum/1c8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8c ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DN72_SULSO DN72_SULSO]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature. Stimulates the Holliday junction cleavage activity of Hjc.<ref>PMID:11709558</ref> | + | [https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sulfolobus solfataricus]] | + | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: Edmondson, S P]] | + | [[Category: Edmondson SP]] |
| - | [[Category: Gao, Y G]] | + | [[Category: Gao Y-G]] |
| - | [[Category: Liaw, Y C]] | + | [[Category: Liaw Y-C]] |
| - | [[Category: Robinson, H]] | + | [[Category: Robinson H]] |
| - | [[Category: Shriver, J W]] | + | [[Category: Shriver JW]] |
| - | [[Category: Su, S]] | + | [[Category: Su S]] |
| - | [[Category: Wang, A H.J]] | + | [[Category: Wang AH-J]] |
| - | [[Category: Achaeabacteria]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Dna binding protein-dna complex]]
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| - | [[Category: Electrostatic]]
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| - | [[Category: Hyperthermophile]]
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| - | [[Category: Molecular modeling]]
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| - | [[Category: Protein stability]]
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| - | [[Category: Protein-dna interaction]]
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| - | [[Category: T-g mismatch]]
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| Structural highlights
Function
DN7D_SACS2 Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990][1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs.,Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kvaratskhelia M, Wardleworth BN, Bond CS, Fogg JM, Lilley DM, White MF. Holliday junction resolution is modulated by archaeal chromatin components in vitro. J Biol Chem. 2002 Jan 25;277(4):2992-6. Epub 2001 Nov 14. PMID:11709558 doi:http://dx.doi.org/10.1074/jbc.M109496200
- ↑ Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH. Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs. J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116 doi:http://dx.doi.org/10.1006/jmbi.2000.4112
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