1d5t

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(New page: 200px<br /><applet load="1d5t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d5t, resolution 1.04&Aring;" /> '''GUANINE NUCLEOTIDE D...)
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[[Image:1d5t.jpg|left|200px]]<br /><applet load="1d5t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d5t, resolution 1.04&Aring;" />
 
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'''GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM'''<br />
 
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==Overview==
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==GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM==
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Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that, functions in vesicular membrane transport to recycle Rab GTPases. We have, now determined the crystal structure of bovine alpha-GDI at ultra-high, resolution (1.04 A). Refinement at this resolution highlighted a region, with high mobility of its main-chain residues. This corresponded to a, surface loop in the primarily alpha-helical domain II at the base of, alpha-GDI containing the previously uncharacterized sequence-conserved, region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop, plays a crucial role in binding of GDI to membranes and extraction of, membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I, at the apex of alpha-GDI may provide flexibility for recycling of diverse, Rab GTPases. We propose that conserved residues in domains I and II, synergize to form the functional face of GDI, and that domain II mediates, a critical step in Rab recycling during vesicle fusion.
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<StructureSection load='1d5t' size='340' side='right'caption='[[1d5t]], [[Resolution|resolution]] 1.04&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1d5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D5T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.04&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d5t OCA], [https://pdbe.org/1d5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d5t RCSB], [https://www.ebi.ac.uk/pdbsum/1d5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d5t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GDIA_BOVIN GDIA_BOVIN] Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d5/1d5t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d5t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine alpha-GDI at ultra-high resolution (1.04 A). Refinement at this resolution highlighted a region with high mobility of its main-chain residues. This corresponded to a surface loop in the primarily alpha-helical domain II at the base of alpha-GDI containing the previously uncharacterized sequence-conserved region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I at the apex of alpha-GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion.
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==About this Structure==
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A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling.,Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA Traffic. 2000 Mar;1(3):270-81. PMID:11208110<ref>PMID:11208110</ref>
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1D5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D5T OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11208110 11208110]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1d5t" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Balch, W.E.]]
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[[Category: Greasley, S.E.]]
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[[Category: Heine, A.]]
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[[Category: Kuhn, P.]]
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[[Category: Moyer, B.]]
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[[Category: Peng, L.]]
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[[Category: Wilson, I.A.]]
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[[Category: Zeng, K.]]
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[[Category: SO4]]
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[[Category: ultra-high resolution]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:01:42 2007''
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==See Also==
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*[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Balch WE]]
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[[Category: Greasley SE]]
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[[Category: Heine A]]
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[[Category: Kuhn P]]
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[[Category: Moyer B]]
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[[Category: Peng L]]
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[[Category: Wilson IA]]
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[[Category: Zeng K]]

Current revision

GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM

PDB ID 1d5t

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