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| | ==LYME DISEASE ANTIGEN OSPA IN COMPLEX WITH NEUTRALIZING ANTIBODY FAB LA-2== | | ==LYME DISEASE ANTIGEN OSPA IN COMPLEX WITH NEUTRALIZING ANTIBODY FAB LA-2== |
| - | <StructureSection load='1fj1' size='340' side='right' caption='[[1fj1]], [[Resolution|resolution]] 2.68Å' scene=''> | + | <StructureSection load='1fj1' size='340' side='right'caption='[[1fj1]], [[Resolution|resolution]] 2.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1fj1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Borbu Borbu] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2osp 2osp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJ1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1fj1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2osp 2osp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJ1 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1osp|1osp]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fj1 OCA], [http://pdbe.org/1fj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fj1 RCSB], [http://www.ebi.ac.uk/pdbsum/1fj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fj1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fj1 OCA], [https://pdbe.org/1fj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fj1 RCSB], [https://www.ebi.ac.uk/pdbsum/1fj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fj1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[G09SecL04Tpc2|G09SecL04Tpc2]] | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| - | *[[G1SecL05|G1SecL05]] | + | *[[Outer surface protein|Outer surface protein]] |
| - | *[[OspA|OspA]]
| + | |
| - | *[[OspA L03 Group2|OspA L03 Group2]]
| + | |
| - | *[[3D structures of monoclonal antibody|3D structures of monoclonal antibody]]
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Borbu]] | + | [[Category: Borreliella burgdorferi B31]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Mus musculus]] | | [[Category: Mus musculus]] |
| - | [[Category: Ding, W]] | + | [[Category: Ding W]] |
| - | [[Category: Lawson, C L]] | + | [[Category: Lawson CL]] |
| - | [[Category: Antibody fab fragment]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Lyme disease]]
| + | |
| - | [[Category: Neutralizing epitope]]
| + | |
| - | [[Category: Ospa]]
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| Structural highlights
Function
IGKC_MOUSE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Outer surface protein A (OspA) is a major lipoprotein of the Borrelia burgdorferi spirochete, the causative agent of Lyme disease. Vaccination with OspA generates an immune response that can prevent bacterial transmission to a mammalian host during the attachment of an infected tick. However, the protective capacity of immune sera cannot be predicted by measuring total anti-OspA antibody. The murine monoclonal antibody LA-2 defines an important protective B-cell epitope of OspA against which protective sera have strong levels of reactivity. We have now mapped the LA-2 epitope of OspA using both NMR chemical-shift perturbation measurements in solution and X-ray crystal structure determination. LA-2 recognizes the three surface-exposed loops of the C-terminal domain of OspA that are on the tip of the elongated molecule most distant from the lipid-modified N terminus. The structure suggests that the natural variation at OspA sequence position 208 in the first loop is a major limiting factor for antibody cross-reactivity between different Lyme disease-causing Borrelia strains. The unusual Fab-dominated lattice of the crystal also permits a rare view of antigen flexibility within an antigen:antibody complex. These results provide a rationale for improvements in OspA-based vaccines and suggest possible designs for more direct tests of antibody protective levels in vaccinated individuals.
Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA.,Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL J Mol Biol. 2000 Oct 6;302(5):1153-64. PMID:11183781[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL. Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J Mol Biol. 2000 Oct 6;302(5):1153-64. PMID:11183781 doi:10.1006/jmbi.2000.4119
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