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1i3t

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[[Image:1i3t.gif|left|200px]]
 
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{{Structure
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==MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA==
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|PDB= 1i3t |SIZE=350|CAPTION= <scene name='initialview01'>1i3t</scene>, resolution 1.6&Aring;
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<StructureSection load='1i3t' size='340' side='right'caption='[[1i3t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=C45:N4-METHOXY-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>C45</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1i3t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I3T FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C45:N4-METHOXY-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C45</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3t OCA], [https://pdbe.org/1i3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i3t RCSB], [https://www.ebi.ac.uk/pdbsum/1i3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i3t ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3t OCA], [http://www.ebi.ac.uk/pdbsum/1i3t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i3t RCSB]</span>
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<div style="background-color:#fffaf0;">
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}}
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== Publication Abstract from PubMed ==
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'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA'''
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==Overview==
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To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.
To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.
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==About this Structure==
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Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex.,Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A J Biochem. 2001 Jul;130(1):9-12. PMID:11432773<ref>PMID:11432773</ref>
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1I3T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3T OCA].
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==Reference==
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Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem. 2001 Jul;130(1):9-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11432773 11432773]
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[[Category: Protein complex]]
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[[Category: Chatake, T.]]
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[[Category: Hikima, T.]]
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[[Category: Hossain, M T.]]
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[[Category: Masaru, T.]]
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[[Category: Matsuda, A.]]
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[[Category: Sunami, T.]]
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[[Category: Takenaka, A.]]
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[[Category: Ueno, Y.]]
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[[Category: b-dna]]
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[[Category: damaged dna]]
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[[Category: deoxyribonucleic acid]]
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[[Category: double helix]]
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[[Category: n4-methoxycytosine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:14:14 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1i3t" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Chatake T]]
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[[Category: Hikima T]]
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[[Category: Hossain MT]]
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[[Category: Masaru T]]
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[[Category: Matsuda A]]
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[[Category: Sunami T]]
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[[Category: Takenaka A]]
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[[Category: Ueno Y]]

Current revision

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA

PDB ID 1i3t

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