1xvr

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(New page: 200px<br /><applet load="1xvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvr, resolution 1.40&Aring;" /> '''echinomycin (CGTACG)...)
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[[Image:1xvr.gif|left|200px]]<br /><applet load="1xvr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xvr, resolution 1.40&Aring;" />
 
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'''echinomycin (CGTACG)2 complex'''<br />
 
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==Overview==
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==echinomycin (CGTACG)2 complex==
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The structure of the bis-intercalation complex of the depsipeptide, antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher, resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are, reported for the complexes of echinomycin with (GCGTACGC)2 (at both low, and high ionic strengths) and (ACGTACGT)2. The structures show the, expected Hoogsteen pairing for the base pairs flanking the intercalating, chromophores on the outside and Watson-Crick pairing for both base pairs, enclosed by the echinomycin. In the octamer complexes but not the hexamer, complex, the echinomycin molecule, which would possess a molecular twofold, axis were it not for the thioacetal bridge, shows twofold disorder. In all, the structures the stacking of the base pairs and chromophores is extended, by intermolecular stacking. The structures provide more precise details of, the hydrogen bonding and other interactions between the bis-intercalating, antibiotics and the duplex DNA than were previously available.
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<StructureSection load='1xvr' size='340' side='right'caption='[[1xvr]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xvr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_echinatus Streptomyces echinatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XVR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=N2C:N,S-DIMETHYLCYSTEINE'>N2C</scene>, <scene name='pdbligand=NCY:N-METHYLCYSTEINE'>NCY</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xvr OCA], [https://pdbe.org/1xvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xvr RCSB], [https://www.ebi.ac.uk/pdbsum/1xvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xvr ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the bis-intercalation complex of the depsipeptide antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are reported for the complexes of echinomycin with (GCGTACGC)2 (at both low and high ionic strengths) and (ACGTACGT)2. The structures show the expected Hoogsteen pairing for the base pairs flanking the intercalating chromophores on the outside and Watson-Crick pairing for both base pairs enclosed by the echinomycin. In the octamer complexes but not the hexamer complex, the echinomycin molecule, which would possess a molecular twofold axis were it not for the thioacetal bridge, shows twofold disorder. In all the structures the stacking of the base pairs and chromophores is extended by intermolecular stacking. The structures provide more precise details of the hydrogen bonding and other interactions between the bis-intercalating antibiotics and the duplex DNA than were previously available.
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==About this Structure==
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Structures of complexes between echinomycin and duplex DNA.,Cuesta-Seijo JA, Sheldrick GM Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:15805599<ref>PMID:15805599</ref>
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1XVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, QUI, DSN and SER as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XVR OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structures of complexes between echinomycin and duplex DNA., Cuesta-Seijo JA, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805599 15805599]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1xvr" style="background-color:#fffaf0;"></div>
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[[Category: Cuesta-Seijo, J.A.]]
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== References ==
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[[Category: Sheldrick, G.M.]]
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<references/>
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[[Category: DSN]]
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__TOC__
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[[Category: MG]]
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</StructureSection>
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[[Category: QUI]]
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[[Category: Large Structures]]
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[[Category: SER]]
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[[Category: Streptomyces echinatus]]
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[[Category: bisintercalator]]
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[[Category: Cuesta-Seijo JA]]
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[[Category: hoogsteen basepairs]]
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[[Category: Sheldrick GM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:02:26 2007''
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Current revision

echinomycin (CGTACG)2 complex

PDB ID 1xvr

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