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| | ==Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus== | | ==Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus== |
| - | <StructureSection load='1yc6' size='340' side='right' caption='[[1yc6]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='1yc6' size='340' side='right'caption='[[1yc6]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1yc6]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Bmv Bmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YC6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1yc6]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Brome_mosaic_virus Brome mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YC6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1js9|1js9]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yc6 OCA], [http://pdbe.org/1yc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yc6 RCSB], [http://www.ebi.ac.uk/pdbsum/1yc6 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yc6 OCA], [https://pdbe.org/1yc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1yc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yc6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CAPSD_BMV CAPSD_BMV]] Capsid protein. Binds specifically to the subgenomic RNA4 to ensure selective packaging. | + | [https://www.uniprot.org/uniprot/CAPSD_BMV CAPSD_BMV] Capsid protein. Binds specifically to the subgenomic RNA4 to ensure selective packaging. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Virus coat protein|Virus coat protein]] | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bmv]] | + | [[Category: Brome mosaic virus]] |
| - | [[Category: Larson, S B]]
| + | [[Category: Large Structures]] |
| - | [[Category: Lucas, R W]]
| + | [[Category: Larson SB]] |
| - | [[Category: McPherson, A]]
| + | [[Category: Lucas RW]] |
| - | [[Category: Almv]]
| + | [[Category: McPherson A]] |
| - | [[Category: Assembly]]
| + | |
| - | [[Category: Icosahedral virus]]
| + | |
| - | [[Category: Proteolysis]] | + | |
| - | [[Category: Structural transition]] | + | |
| - | [[Category: Symmetry]] | + | |
| - | [[Category: Virus]] | + | |
| Structural highlights
Function
CAPSD_BMV Capsid protein. Binds specifically to the subgenomic RNA4 to ensure selective packaging.
Publication Abstract from PubMed
T=1 icosahedral particles of amino terminally truncated brome mosaic virus (BMV) protein were created by treatment of the wild-type T=3 virus with 1M CaCl2 and crystallized from sodium malonate. Diffraction data were collected from frozen crystals to beyond 2.9 A resolution and the structure determined by molecular replacement and phase extension. The particles are composed of pentameric capsomeres from the wild-type virions which have reoriented with respect to the original particle pentameric axes by rotations of 37 degrees , and formed tenuous interactions with one another, principally through conformationally altered C-terminal polypeptides. Otherwise, the pentamers are virtually superimposable upon those of the original T=3 BMV particles. The T=1 particles, in the crystals, are not perfect icosahedra, but deviate slightly from exact symmetry, possibly due to packing interactions. This suggests that the T=1 particles are deformable, which is consistent with the loose arrangement of pentamers and latticework of holes that penetrate the surface. Atomic force microscopy showed that the T=3 to T=1 transition could occur by shedding of hexameric capsomeres and restructuring of remaining pentamers accompanied by direct condensation. Knowledge of the structures of the BMV wild-type and T=1 particles now permit us to propose a tentative model for that process. A comparison of the BMV T=1 particles was made with the reassembled T=1 particles produced from the coat protein of trypsin treated alfalfa mosaic virus (AlMV), another bromovirus. There is little resemblance between the two particles. The BMV particle, with a maximum diameter of 195 A, is made from distinctive pentameric capsomeres with large holes along the 3-fold axis, while the AlMV particle, of approximate maximum diameter 220 A, has subunits closely packed around the 3-fold axis, large holes along the 5-fold axis, and few contacts within pentamers. In both particles crucial linkages are made about icosahedral dyads.
Crystallographic structure of the T=1 particle of brome mosaic virus.,Larson SB, Lucas RW, McPherson A J Mol Biol. 2005 Feb 25;346(3):815-31. Epub 2005 Jan 12. PMID:15713465[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Larson SB, Lucas RW, McPherson A. Crystallographic structure of the T=1 particle of brome mosaic virus. J Mol Biol. 2005 Feb 25;346(3):815-31. Epub 2005 Jan 12. PMID:15713465 doi:S0022-2836(04)01585-2
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