1zik

From Proteopedia

(Difference between revisions)

Revision as of 07:15, 9 August 2023

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE

Structural highlights

1zik is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GCN4_YEAST Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.

Publication Abstract from PubMed

A conserved asparagine (Asn 16) buried in the interface of the GCN4 leucine zipper selectively favours the parallel, dimeric, coiled-coil structure. To test if other polar residues confer oligomerization specificity, the structural effects of Gln and Lys substitutions for Asn 16 were characterized. Like the wild-type peptide, the Asn 16Lys mutant formed exclusively dimers. In contrast, Gln 16, despite its chemical similarity to Asn, allowed the peptide to form both dimers and trimers. The Gln 16 side chain was accommodated by qualitatively different interactions in the dimer and trimer crystal structures. These findings demonstrate that the structural selectivity of polar residues results not only from the burial of polar atoms, but also depends on the complementarity of the side-chain stereochemistry with the surrounding structural environment.

Buried polar residues and structural specificity in the GCN4 leucine zipper.,Gonzalez L Jr, Woolfson DN, Alber T Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:8946854^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gonzalez L Jr, Woolfson DN, Alber T. Buried polar residues and structural specificity in the GCN4 leucine zipper. Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:8946854

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zik"

Categories: Large Structures | Saccharomyces cerevisiae | Alber T | Gonzalez Junior L | Woolfson DN

@@ Line 3: / Line 3: @@
 <StructureSection load='1zik' size='340' side='right'caption='[[1zik]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1zik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1zik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zik OCA], [https://pdbe.org/1zik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zik RCSB], [https://www.ebi.ac.uk/pdbsum/1zik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zik ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zik OCA], [https://pdbe.org/1zik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zik RCSB], [https://www.ebi.ac.uk/pdbsum/1zik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zik ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
+[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 20: @@
 ==See Also==
+*[[Gcn4 3D Structures|Gcn4 3D Structures]]
 *[[Gnc4 3D Structures|Gnc4 3D Structures]]
 == References ==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Alber, T]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Junior, L Gonzalez]]
+[[Category: Alber T]]
-[[Category: Woolfson, D N]]
+[[Category: Gonzalez Junior L]]
-[[Category: Activator]]
+[[Category: Woolfson DN]]
-[[Category: Amino-acid biosynthesis]]
-[[Category: Coiled coil]]
-[[Category: Dna-binding]]
-[[Category: Leucine zipper]]
-[[Category: Nuclear protein]]
-[[Category: Transcription regulation]]

1zik

From Proteopedia

Revision as of 07:15, 9 August 2023

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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