This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2w1t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:16, 9 August 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2w1t.png|left|200px]]
 
-
<!--
+
==Crystal Structure of B. subtilis SpoVT==
-
The line below this paragraph, containing "STRUCTURE_2w1t", creates the "Structure Box" on the page.
+
<StructureSection load='2w1t' size='340' side='right'caption='[[2w1t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2w1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1T FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1t OCA], [https://pdbe.org/2w1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1t RCSB], [https://www.ebi.ac.uk/pdbsum/2w1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1t ProSAT]</span></td></tr>
-
{{STRUCTURE_2w1t| PDB=2w1t | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SPOVT_BACSU SPOVT_BACSU] Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).<ref>PMID:22522895</ref> <ref>PMID:8755877</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the crystal structure of the isolated C-terminal domain of SpoVT at 1.5 A by experimental phasing techniques and used this model to solve the structure of the full-length SpoVT at 2.6 A by molecular replacement. SpoVT is a tetramer that shows an overall significant distortion mediated by electrostatic interactions. Two monomers dimerize via the highly charged N-terminal domains to form swapped-hairpin beta-barrels. These asymmetric dimers further tetramerize through the formation of mixed helix bundles between their C-terminal domains, which themselves fold as GAF (cGMP-specific and cGMP-stimulated phosphodiesterases, Anabaena adenylate cyclases, and Escherichia coli FhlA) domains. The combination of a swapped-hairpin beta-barrel with a GAF domain represents a novel domain architecture in transcription factors. The occurrence of SpoVT homologs throughout Bacilli and Clostridia demonstrates the ancestral origin of this factor in sporulation.
-
===CRYSTAL STRUCTURE OF B. SUBTILIS SPOVT===
+
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis.,Asen I, Djuranovic S, Lupas AN, Zeth K J Mol Biol. 2008 Oct 30. PMID:18996130<ref>PMID:18996130</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18996130}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2w1t" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18996130 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18996130}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2W1T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1T OCA].
+
-
 
+
-
==Reference==
+
-
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis., Asen I, Djuranovic S, Lupas AN, Zeth K, J Mol Biol. 2008 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18996130 18996130]
+
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
+
[[Category: Asen I]]
-
[[Category: Activator]]
+
[[Category: Djuranovic S]]
-
[[Category: Dna-binding]]
+
[[Category: Lupas AN]]
-
[[Category: Gaf domain regulated tetrameric spovt]]
+
[[Category: Zeth K]]
-
[[Category: Repressor]]
+
-
[[Category: Sporulation]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 20:22:53 2008''
+

Current revision

Crystal Structure of B. subtilis SpoVT

PDB ID 2w1t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w1t"
Personal tools