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2x3a

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[[Image:2x3a.jpg|left|200px]]
 
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==AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase==
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The line below this paragraph, containing "STRUCTURE_2x3a", creates the "Structure Box" on the page.
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<StructureSection load='2x3a' size='340' side='right'caption='[[2x3a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x3a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2x3a| PDB=2x3a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3a OCA], [https://pdbe.org/2x3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3a RCSB], [https://www.ebi.ac.uk/pdbsum/2x3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GMV9_AERSA Q8GMV9_AERSA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Gram-negative bacterium Aeromonas salmonicida is a fish pathogen for various fish species worldwide. Aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase AsaP1. Crystal structure analyses at 2.0 A resolution of two proteolytically inactive AsaP1 variants show the polypeptide folding of the protease domain and the propeptide domain. These first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into propeptide function, and specific substrate binding. A lysine side chain of the propeptide binds in the hydrophobic S1'-pocket interacting with three carboxylate side chains. An AsaP1 variant with a lysine to alanine exchange identifies the chaperone function of the propeptide.
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===ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===
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Structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase AsaP1.,Bogdanovic X, Palm GJ, Schwenteit J, Singh RK, Gudmundsdottir BK, Hinrichs W FEBS Lett. 2016 Aug 16. doi: 10.1002/1873-3468.12356. PMID:27528449<ref>PMID:27528449</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2x3a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3A OCA].
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<div class="pdbe-citations 2x3a" style="background-color:#fffaf0;"></div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:19574642</ref><ref group="xtra">PMID:18952802</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Aeromonas salmonicida subsp. achromogenes]]
[[Category: Aeromonas salmonicida subsp. achromogenes]]
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[[Category: Deuterolysin]]
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[[Category: Large Structures]]
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[[Category: Bogdanovic, X.]]
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[[Category: Bogdanovic X]]
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[[Category: Hinrichs, W.]]
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[[Category: Hinrichs W]]
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[[Category: Palm, G J.]]
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[[Category: Palm GJ]]
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[[Category: Singh, R K.]]
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[[Category: Singh RK]]

Current revision

AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase

PDB ID 2x3a

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