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5h1s
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5h1s is ON HOLD Authors: Ahmed, T, Yin, Z, Bhushan, S Description: Structure of the large subunit of the chloro-ribosome [[Category: Unreleased Str...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the large subunit of the chloro-ribosome== | |
| + | <SX load='5h1s' size='340' side='right' viewer='molstar' caption='[[5h1s]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5h1s]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H1S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1s OCA], [https://pdbe.org/5h1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h1s RCSB], [https://www.ebi.ac.uk/pdbsum/5h1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RK13_SPIOL RK13_SPIOL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein synthesis in the chloroplast is mediated by the chloroplast ribosome (chloro-ribosome). Overall architecture of the chloro-ribosome is considerably similar to the Escherichia coli (E. coli) ribosome but certain differences are evident. The chloro-ribosome proteins are generally larger because of the presence of chloroplast-specific extensions in their N- and C-termini. The chloro-ribosome harbours six plastid-specific ribosomal proteins (PSRPs); four in the small subunit and two in the large subunit. Deletions and insertions occur throughout the rRNA sequence of the chloro-ribosome (except for the conserved peptidyl transferase center region) but the overall length of the rRNAs do not change significantly, compared to the E. coli. Although, recent advancements in cryo-electron microscopy (cryo-EM) have provided detailed high-resolution structures of ribosomes from many different sources, a high-resolution structure of the chloro-ribosome is still lacking. Here, we present a cryo-EM structure of the large subunit of the chloro-ribosome from spinach (Spinacia oleracea) at an average resolution of 3.5 A. High-resolution map enabled us to localize and model chloro-ribosome proteins, chloroplast-specific protein extensions, two PSRPs (PSRP5 and 6) and three rRNA molecules present in the chloro-ribosome. Although comparable to E. coli, the polypeptide tunnel and the tunnel exit site show chloroplast-specific features. | ||
| - | + | Cryo-EM structure of the large subunit of the spinach chloroplast ribosome.,Ahmed T, Yin Z, Bhushan S Sci Rep. 2016 Oct 20;6:35793. doi: 10.1038/srep35793. PMID:27762343<ref>PMID:27762343</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Ahmed | + | <div class="pdbe-citations 5h1s" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Spinacia oleracea]] | ||
| + | [[Category: Ahmed T]] | ||
| + | [[Category: Bhushan S]] | ||
| + | [[Category: Yin Z]] | ||
Current revision
Structure of the large subunit of the chloro-ribosome
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