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| | ==Crystal structure of the karyopherin Kap121p bound to the SUMO protease Ulp1p== | | ==Crystal structure of the karyopherin Kap121p bound to the SUMO protease Ulp1p== |
| - | <StructureSection load='5h2v' size='340' side='right' caption='[[5h2v]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='5h2v' size='340' side='right'caption='[[5h2v]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h2v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H2V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H2V FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h2x|5h2x]], [[5h2w|5h2w]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ulp1_peptidase Ulp1 peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.68 3.4.22.68] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2v OCA], [https://pdbe.org/5h2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h2v RCSB], [https://www.ebi.ac.uk/pdbsum/5h2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2v ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2v OCA], [http://pdbe.org/5h2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h2v RCSB], [http://www.ebi.ac.uk/pdbsum/5h2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IMB3_YEAST IMB3_YEAST]] Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). Plays a role in protein secretion. [[http://www.uniprot.org/uniprot/ULP1_YEAST ULP1_YEAST]] Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.<ref>PMID:10094048</ref> | + | [https://www.uniprot.org/uniprot/IMB3_YEAST IMB3_YEAST] Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). Plays a role in protein secretion. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5h2v" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5h2v" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Importin 3D structures|Importin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ulp1 peptidase]] | + | [[Category: Large Structures]] |
| - | [[Category: Kobayashi, J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Matsuura, Y]] | + | [[Category: Kobayashi J]] |
| - | [[Category: Nuclear import]] | + | [[Category: Matsuura Y]] |
| - | [[Category: Protein transport-hydrolase complex]]
| + | |
| Structural highlights
Function
IMB3_YEAST Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). Plays a role in protein secretion.
Publication Abstract from PubMed
The budding yeast small ubiquitin-like modifier (SUMO) protease Ulp1p catalyzes both the processing of newly synthesized SUMO to its mature form and the deconjugation of SUMO from target proteins, thereby regulating a wide range of cellular processes including cell division, DNA repair, DNA replication, transcription, and mRNA quality control. Ulp1p is localized primarily at the nuclear pore complex (NPC) through interactions involving the karyopherins Kap121p and Kap95p-Kap60p heterodimer and a subset of nuclear pore-associated proteins. The sequestration of Ulp1p at the nuclear periphery is crucial for the proper control of protein desumoylation. To gain insights into the role of the karyopherins in regulating the localization of Ulp1p, we have determined the crystal structures of Kap121p and Kap60p bound to the N-terminal non-catalytic domain of Ulp1p that is necessary and sufficient for NPC targeting. Contrary to a previous proposal that Ulp1p is tethered to the transport channel of the NPC through unconventional interactions with the karyopherins, our structures reveal that Ulp1p has canonical nuclear localization signals (NLSs): (1) an isoleucine-lysine-NLS (residues 51-55) that binds to the NLS-binding site of Kap121p, and (2) a classical bipartite NLS (residues 154-172) that binds to the major and minor NLS-binding sites of Kap60p. Ulp1p also binds Kap95p directly, and the Ulp1p-Kap95p binding is enhanced by the importin-beta-binding domain of Kap60p. GTP-bound Gsp1p (the yeast Ran ortholog) and the exportin Cse1p cooperate to release Ulp1p from the karyopherins, indicating that the stable sequestration of Ulp1p to the NPC would require a karyopherin-independent mechanism to anchor Ulp1p at the NPC.
Structures of the Karyopherins Kap121p and Kap60p Bound to the Nuclear Pore-Targeting Domain of the SUMO Protease Ulp1p.,Hirano H, Kobayashi J, Matsuura Y J Mol Biol. 2017 Jan 20;429(2):249-260. doi: 10.1016/j.jmb.2016.11.029. Epub 2016, Dec 6. PMID:27939291[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hirano H, Kobayashi J, Matsuura Y. Structures of the Karyopherins Kap121p and Kap60p Bound to the Nuclear Pore-Targeting Domain of the SUMO Protease Ulp1p. J Mol Biol. 2017 Jan 20;429(2):249-260. doi: 10.1016/j.jmb.2016.11.029. Epub 2016, Dec 6. PMID:27939291 doi:http://dx.doi.org/10.1016/j.jmb.2016.11.029
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