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| | <StructureSection load='5hj0' size='340' side='right'caption='[[5hj0]], [[Resolution|resolution]] 2.64Å' scene=''> | | <StructureSection load='5hj0' size='340' side='right'caption='[[5hj0]], [[Resolution|resolution]] 2.64Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hj0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HJ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hj0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HJ0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mis18, SPCC970.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hj0 OCA], [http://pdbe.org/5hj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hj0 RCSB], [http://www.ebi.ac.uk/pdbsum/5hj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hj0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hj0 OCA], [https://pdbe.org/5hj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hj0 RCSB], [https://www.ebi.ac.uk/pdbsum/5hj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hj0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MIS18_SCHPO MIS18_SCHPO]] Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere. Has a role in kinetochore assembly and CENP-A loading. Maintains the deacetylated state of histones specifically in the central core of the centromeres.<ref>PMID:15369671</ref> | + | [https://www.uniprot.org/uniprot/MIS18_SCHPO MIS18_SCHPO] Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere. Has a role in kinetochore assembly and CENP-A loading. Maintains the deacetylated state of histones specifically in the central core of the centromeres.<ref>PMID:15369671</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cbs 356]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Allshire, R]] | + | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Jeyaprakash, A Arockia]] | + | [[Category: Allshire R]] |
| - | [[Category: Medina-Pritchard, B]] | + | [[Category: Arockia Jeyaprakash A]] |
| - | [[Category: Subramanian, L]] | + | [[Category: Medina-Pritchard B]] |
| - | [[Category: Centromere]]
| + | [[Category: Subramanian L]] |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Mis18]]
| + | |
| Structural highlights
Function
MIS18_SCHPO Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere. Has a role in kinetochore assembly and CENP-A loading. Maintains the deacetylated state of histones specifically in the central core of the centromeres.[1]
Publication Abstract from PubMed
Mis18 is a key regulator responsible for the centromere localization of the CENP-A chaperone Scm3 in Schizosaccharomyces pombe and HJURP in humans, which establishes CENP-A chromatin that defines centromeres. The molecular and structural determinants of Mis18 centromere targeting remain elusive. Here, by combining structural, biochemical, and yeast genetic studies, we show that the oligomerization of S. pombe Mis18, mediated via its conserved N-terminal Yippee-like domain, is crucial for its centromere localization and function. The crystal structure of the N-terminal Yippee-like domain reveals a fold containing a cradle-shaped pocket that is implicated in protein/nucleic acid binding, which we show is required for Mis18 function. While the N-terminal Yippee-like domain forms a homodimer in vitro and in vivo, full-length Mis18, including the C-terminal alpha-helical domain, forms a homotetramer in vitro. We also show that the Yippee-like domains of human Mis18alpha/Mis18beta interact to form a heterodimer, implying a conserved structural theme for Mis18 regulation.
Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization.,Subramanian L, Medina-Pritchard B, Barton R, Spiller F, Kulasegaran-Shylini R, Radaviciute G, Allshire RC, Arockia Jeyaprakash A EMBO Rep. 2016 Feb 26. pii: e201541520. PMID:26921242[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell. 2004 Sep 17;118(6):715-29. PMID:15369671 doi:http://dx.doi.org/10.1016/j.cell.2004.09.002
- ↑ Subramanian L, Medina-Pritchard B, Barton R, Spiller F, Kulasegaran-Shylini R, Radaviciute G, Allshire RC, Arockia Jeyaprakash A. Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization. EMBO Rep. 2016 Feb 26. pii: e201541520. PMID:26921242 doi:http://dx.doi.org/10.15252/embr.201541520
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