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| | ==TonEBP/DNA COMPLEX== | | ==TonEBP/DNA COMPLEX== |
| - | <StructureSection load='1imh' size='340' side='right' caption='[[1imh]], [[Resolution|resolution]] 2.86Å' scene=''> | + | <StructureSection load='1imh' size='340' side='right'caption='[[1imh]], [[Resolution|resolution]] 2.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1imh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1imh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMH FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NFAT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.86Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imh OCA], [http://pdbe.org/1imh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imh RCSB], [http://www.ebi.ac.uk/pdbsum/1imh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1imh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imh OCA], [https://pdbe.org/1imh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imh RCSB], [https://www.ebi.ac.uk/pdbsum/1imh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NFAT5_HUMAN NFAT5_HUMAN]] Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. | + | [https://www.uniprot.org/uniprot/NFAT5_HUMAN NFAT5_HUMAN] Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Chen, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Lopez-Rodriguez, C]] | + | [[Category: Chen L]] |
| - | [[Category: Rao, A]] | + | [[Category: Lopez-Rodriguez C]] |
| - | [[Category: Stroud, J C]] | + | [[Category: Rao A]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Stroud JC]] |
| - | [[Category: Dna encirclement]]
| + | |
| - | [[Category: Double helix]]
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| - | [[Category: Protein-dna complex]]
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| - | [[Category: Transcription-dna complex]]
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| Structural highlights
Function
NFAT5_HUMAN Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Tonicity-responsive enhancer binding protein (TonEBP), also known as NFAT5, is a unique member of the NFAT family of transcription factors that regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of the NFAT family, TonEBP exists as a homodimer and binds asymmetric TonE DNA sites; furthermore, the affinity of TonEBP for DNA is much lower than that of other NFAT proteins. How TonEBP recognizes the TonE site and regulates the activation of hypertonicity response genes has not been clear. Here we show that TonEBP adopts a NF-kappaB-like structure upon binding to DNA, providing a direct structural link between the NFAT and NF-kappaB family of transcription factors. We also show that TonEBP completely encircles its DNA target and present biochemical evidence that the DNA encirclement may lead to increased kinetic stability of the TonEBP-DNA complex. Thus, the list of proteins that bind DNA by encirclement is now expanded to include sequence-specific transcription factors.
Structure of a TonEBP-DNA complex reveals DNA encircled by a transcription factor.,Stroud JC, Lopez-Rodriguez C, Rao A, Chen L Nat Struct Biol. 2002 Feb;9(2):90-4. PMID:11780147[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stroud JC, Lopez-Rodriguez C, Rao A, Chen L. Structure of a TonEBP-DNA complex reveals DNA encircled by a transcription factor. Nat Struct Biol. 2002 Feb;9(2):90-4. PMID:11780147 doi:10.1038/nsb749
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