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1keq

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(New page: 200px<br /><applet load="1keq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1keq, resolution 1.88&Aring;" /> '''Crystal Structure of...)
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[[Image:1keq.jpg|left|200px]]<br /><applet load="1keq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1keq, resolution 1.88&Aring;" />
 
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'''Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole'''<br />
 
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==Overview==
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==Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole==
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The crystal structure of F65A/Y131C murine alpha-carbonic anhydrase V, (CAV), covalently modified at cysteine residues with, 4-chloromethylimidazole, is reported at 1.88 A resolution. This, modification introduces a methylimidazole (MI) group at residue C131 in, the active site with important consequences. F65A/Y131C-MI CAV exhibits an, up to 3-fold enhancement of catalytic activity over that of wild-type CAV, [Earnhardt, J. N., Wright, S. K., Qian, M., Tu, C., Laipis, P. J., Viola, R. E., and Silverman, D. N. (1999) Arch. Biochem. Biophys. 361, 264-270]., In this modified CAV variant, C131-MI acts as a proton shuttle, facilitating the deprotonation of a zinc-bound water molecule to, regenerate the nucleophilic zinc-bound hydroxide ion. A network of three, hydrogen-bonded water molecules, across which proton transfer likely, proceeds, bridges the zinc-bound water molecule and the C131-MI imidazole, group. The structure of F65A/Y131C-MI CAV is compared to structures of, Y64H/F65A murine CAV, wild-type human alpha-carbonic anhydrase II, and the, gamma-carbonic anhydrase from Methanosarcina thermophilain an effort to, outline common features of catalytic proton shuttles.
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<StructureSection load='1keq' size='340' side='right'caption='[[1keq]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1keq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KEQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MZ:4-METHYLIMIDAZOLE'>4MZ</scene>, <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1keq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1keq OCA], [https://pdbe.org/1keq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1keq RCSB], [https://www.ebi.ac.uk/pdbsum/1keq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1keq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAH5A_MOUSE CAH5A_MOUSE] Reversible hydration of carbon dioxide. Low activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/1keq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1keq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of F65A/Y131C murine alpha-carbonic anhydrase V (CAV), covalently modified at cysteine residues with 4-chloromethylimidazole, is reported at 1.88 A resolution. This modification introduces a methylimidazole (MI) group at residue C131 in the active site with important consequences. F65A/Y131C-MI CAV exhibits an up to 3-fold enhancement of catalytic activity over that of wild-type CAV [Earnhardt, J. N., Wright, S. K., Qian, M., Tu, C., Laipis, P. J., Viola, R. E., and Silverman, D. N. (1999) Arch. Biochem. Biophys. 361, 264-270]. In this modified CAV variant, C131-MI acts as a proton shuttle, facilitating the deprotonation of a zinc-bound water molecule to regenerate the nucleophilic zinc-bound hydroxide ion. A network of three hydrogen-bonded water molecules, across which proton transfer likely proceeds, bridges the zinc-bound water molecule and the C131-MI imidazole group. The structure of F65A/Y131C-MI CAV is compared to structures of Y64H/F65A murine CAV, wild-type human alpha-carbonic anhydrase II, and the gamma-carbonic anhydrase from Methanosarcina thermophilain an effort to outline common features of catalytic proton shuttles.
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==About this Structure==
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Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle.,Jude KM, Wright SK, Tu C, Silverman DN, Viola RE, Christianson DW Biochemistry. 2002 Feb 26;41(8):2485-91. PMID:11851394<ref>PMID:11851394</ref>
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1KEQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with K, ZN, 4MZ and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KEQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle., Jude KM, Wright SK, Tu C, Silverman DN, Viola RE, Christianson DW, Biochemistry. 2002 Feb 26;41(8):2485-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11851394 11851394]
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</div>
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[[Category: Carbonate dehydratase]]
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<div class="pdbe-citations 1keq" style="background-color:#fffaf0;"></div>
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[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Christianson, D.W.]]
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[[Category: Jude, K.M.]]
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[[Category: Silverman, D.N.]]
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[[Category: Tu, C.]]
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[[Category: Viola, R.E.]]
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[[Category: Wright, S.K.]]
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[[Category: 4MZ]]
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[[Category: ACY]]
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[[Category: K]]
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[[Category: ZN]]
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[[Category: engineered residue]]
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[[Category: proton transfer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:08:13 2007''
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==See Also==
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*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Christianson DW]]
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[[Category: Jude KM]]
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[[Category: Silverman DN]]
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[[Category: Tu C]]
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[[Category: Viola RE]]
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[[Category: Wright SK]]

Current revision

Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole

PDB ID 1keq

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