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1ku8

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[[Image:1ku8.gif|left|200px]]
 
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==Crystal structure of Jacalin==
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The line below this paragraph, containing "STRUCTURE_1ku8", creates the "Structure Box" on the page.
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<StructureSection load='1ku8' size='340' side='right'caption='[[1ku8]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ku8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KU8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ku8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ku8 OCA], [https://pdbe.org/1ku8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ku8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ku8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ku8 ProSAT]</span></td></tr>
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{{STRUCTURE_1ku8| PDB=1ku8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1ku8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ku8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
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'''Crystal structure of Jacalin'''
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Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose.,Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090<ref>PMID:11988090</ref>
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==Overview==
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Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1KU8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU8 OCA].
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</div>
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<div class="pdbe-citations 1ku8" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11988090 11988090]
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Artocarpus integer]]
[[Category: Artocarpus integer]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Astoul, C H.]]
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[[Category: Astoul CH]]
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[[Category: Barre, A.]]
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[[Category: Barre A]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne Y]]
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[[Category: Damme, E J.M Van.]]
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[[Category: Menu-Bouaouiche L]]
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[[Category: Menu-Bouaouiche, L.]]
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[[Category: Peumans WJ]]
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[[Category: Peumans, W J.]]
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[[Category: Rouge P]]
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[[Category: Rouge, P.]]
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[[Category: Van Damme EJM]]
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[[Category: Zamboni, V.]]
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[[Category: Zamboni V]]
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[[Category: Beta-prism fold]]
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[[Category: Carbohydrate binding]]
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[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:10:41 2008''
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Current revision

Crystal structure of Jacalin

PDB ID 1ku8

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