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1kuj

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(New page: 200px<br /><applet load="1kuj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kuj, resolution 2.00&Aring;" /> '''Crystal structure of...)
Current revision (09:04, 16 August 2023) (edit) (undo)
 
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[[Image:1kuj.gif|left|200px]]<br /><applet load="1kuj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kuj, resolution 2.00&Aring;" />
 
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'''Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose'''<br />
 
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==Overview==
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==Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose==
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Evidence is presented that the specificity of jacalin, the seed lectin, from jack fruit (Artocarpus integrifolia), is not directed exclusively, against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and, galactose, but also against mannose and oligomannosides. Biochemical, analyses based on surface-plasmon-resonance measurements, combined with, the X-ray-crystallographic determination of the structure of a, jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated, clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and, N-acetylmuramic acid. Structural analyses demonstrated that the relatively, large size of the carbohydrate-binding site enables jacalin to accommodate, monosaccharides with different hydroxyl conformations and provided, unambiguous evidence that the beta-prism structure of jacalin is a, sufficiently flexible structural scaffold to confer different, carbohydrate-binding specificities to a single lectin.
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<StructureSection load='1kuj' size='340' side='right'caption='[[1kuj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kuj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KUJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kuj OCA], [https://pdbe.org/1kuj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kuj RCSB], [https://www.ebi.ac.uk/pdbsum/1kuj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kuj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1kuj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kuj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
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==About this Structure==
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Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose.,Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090<ref>PMID:11988090</ref>
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1KUJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with MMA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11988090 11988090]
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</div>
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[[Category: Artocarpus integer]]
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<div class="pdbe-citations 1kuj" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Astoul, C.H.]]
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[[Category: Barre, A.]]
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[[Category: Bourne, Y.]]
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[[Category: Damme, E.J.M.Van.]]
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[[Category: Menu-Bouaouiche, L.]]
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[[Category: Peumans, W.J.]]
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[[Category: Rouge, P.]]
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[[Category: Zamboni, V.]]
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[[Category: MMA]]
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[[Category: beta-prism fold]]
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[[Category: carbohydrate binding]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:53:20 2007''
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==See Also==
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Artocarpus integer]]
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[[Category: Large Structures]]
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[[Category: Astoul CH]]
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[[Category: Barre A]]
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[[Category: Bourne Y]]
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[[Category: Menu-Bouaouiche L]]
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[[Category: Peumans WJ]]
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[[Category: Rouge P]]
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[[Category: Van Damme EJM]]
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[[Category: Zamboni V]]

Current revision

Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose

PDB ID 1kuj

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