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1l4a
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="1l4a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l4a, resolution 2.95Å" /> '''X-RAY STRUCTURE OF T...) |
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| - | [[Image:1l4a.gif|left|200px]]<br /><applet load="1l4a" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1l4a, resolution 2.95Å" /> | ||
| - | '''X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI'''<br /> | ||
| - | == | + | ==X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI== |
| - | Nerve terminals release neurotransmitters from vesicles into the synaptic | + | <StructureSection load='1l4a' size='340' side='right'caption='[[1l4a]], [[Resolution|resolution]] 2.95Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1l4a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L4A FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l4a OCA], [https://pdbe.org/1l4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l4a RCSB], [https://www.ebi.ac.uk/pdbsum/1l4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l4a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SYB_DORPE SYB_DORPE] Intrinsic membrane protein of small synaptic vesicles. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l4/1l4a_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l4a ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nerve terminals release neurotransmitters from vesicles into the synaptic cleft upon transient increases in intracellular Ca(2+). This exocytotic process requires the formation of trans SNARE complexes and is regulated by accessory proteins including the complexins. Here we report the crystal structure of a squid core complexin-SNARE complex at 2.95-A resolution. A helical segment of complexin binds in anti-parallel fashion to the four-helix bundle of the core SNARE complex and interacts at its C terminus with syntaxin and synaptobrevin around the ionic zero layer of the SNARE complex. We propose that this structure is part of a multiprotein fusion machinery that regulates vesicle fusion at a late pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion. | ||
| - | + | X-ray structure of a neuronal complexin-SNARE complex from squid.,Bracher A, Kadlec J, Betz H, Weissenhorn W J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:12004067<ref>PMID:12004067</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1l4a" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Syntaxin 3D structures|Syntaxin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Doryteuthis pealeii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Betz H]] | ||
| + | [[Category: Bracher A]] | ||
| + | [[Category: Kadlec J]] | ||
| + | [[Category: Weissenhorn W]] | ||
Current revision
X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI
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