1l5h

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(New page: 200px<br /><applet load="1l5h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l5h, resolution 2.3&Aring;" /> '''FeMo-cofactor Deficie...)
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[[Image:1l5h.jpg|left|200px]]<br /><applet load="1l5h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l5h, resolution 2.3&Aring;" />
 
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'''FeMo-cofactor Deficient Nitrogenase MoFe Protein'''<br />
 
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==Overview==
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==FeMo-cofactor Deficient Nitrogenase MoFe Protein==
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One of the most complex biosynthetic processes in metallobiochemistry is, the assembly of nitrogenase, the key enzyme in biological nitrogen, fixation. We describe here the crystal structure of an iron-molybdenum, cofactor-deficient form of the nitrogenase MoFe protein, into which the, cofactor is inserted in the final step of MoFe protein assembly. The MoFe, protein folds as a heterotetramer containing two copies each of the, homologous alpha and beta subunits. In this structure, one of the three, alpha subunit domains exhibits a substantially changed conformation, whereas the rest of the protein remains essentially unchanged. A, predominantly positively charged funnel is revealed; this funnel is of, sufficient size to accommodate insertion of the negatively charged, cofactor.
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<StructureSection load='1l5h' size='340' side='right'caption='[[1l5h]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CLF:FE(8)-S(7)+CLUSTER'>CLF</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5h OCA], [https://pdbe.org/1l5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5h RCSB], [https://www.ebi.ac.uk/pdbsum/1l5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIFD_AZOVI NIFD_AZOVI] This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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One of the most complex biosynthetic processes in metallobiochemistry is the assembly of nitrogenase, the key enzyme in biological nitrogen fixation. We describe here the crystal structure of an iron-molybdenum cofactor-deficient form of the nitrogenase MoFe protein, into which the cofactor is inserted in the final step of MoFe protein assembly. The MoFe protein folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits. In this structure, one of the three alpha subunit domains exhibits a substantially changed conformation, whereas the rest of the protein remains essentially unchanged. A predominantly positively charged funnel is revealed; this funnel is of sufficient size to accommodate insertion of the negatively charged cofactor.
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==About this Structure==
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Structure of a cofactor-deficient nitrogenase MoFe protein.,Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK Science. 2002 Apr 12;296(5566):352-6. PMID:11951047<ref>PMID:11951047</ref>
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1L5H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with CA and CLF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L5H OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of a cofactor-deficient nitrogenase MoFe protein., Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK, Science. 2002 Apr 12;296(5566):352-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11951047 11951047]
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</div>
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[[Category: Azotobacter vinelandii]]
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<div class="pdbe-citations 1l5h" style="background-color:#fffaf0;"></div>
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[[Category: Nitrogenase]]
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[[Category: Protein complex]]
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[[Category: Burgess, B.K.]]
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[[Category: Dean, D.R.]]
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[[Category: Einsle, O.]]
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[[Category: Rees, D.C.]]
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[[Category: Ribbe, M.W.]]
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[[Category: Schmid, B.]]
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[[Category: Thomas, L.M.]]
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[[Category: Yoshida, M.]]
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[[Category: CA]]
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[[Category: CLF]]
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[[Category: apo-protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:16:52 2007''
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==See Also==
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*[[Nitrogenase 3D structures|Nitrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Azotobacter vinelandii]]
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[[Category: Large Structures]]
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[[Category: Burgess BK]]
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[[Category: Dean DR]]
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[[Category: Einsle O]]
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[[Category: Rees DC]]
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[[Category: Ribbe MW]]
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[[Category: Schmid B]]
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[[Category: Thomas LM]]
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[[Category: Yoshida M]]

Current revision

FeMo-cofactor Deficient Nitrogenase MoFe Protein

PDB ID 1l5h

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