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1lom

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(New page: 200px<br /> <applet load="1lom" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lom, resolution 1.72&Aring;" /> '''CYANOVIRIN-N DOUBLE...)
Current revision (09:16, 16 August 2023) (edit) (undo)
 
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[[Image:1lom.gif|left|200px]]<br />
 
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<applet load="1lom" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lom, resolution 1.72&Aring;" />
 
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'''CYANOVIRIN-N DOUBLE MUTANT P51S S52P'''<br />
 
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==Overview==
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==CYANOVIRIN-N DOUBLE MUTANT P51S S52P==
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Cyanovirin-N (CV-N) is a potent 11 kDa HIV-inactivating protein that binds, with high affinity to the HIV surface envelope protein gp120. A double, mutant P51S/S52P of CV-N was engineered by swapping two critical, hinge-region residues Pro51 and Ser52. This mutant has biochemical and, biophysical characteristics equivalent to the wild-type CV-N and its, structure resembles that of wild-type CV-N. However, the mutant shows a, different orientation in the hinge region that connects two domains of the, protein. The observation that this double mutant crystallizes under a wide, variety of conditions challenges some of the current hypotheses on domain, swapping and on the role of hinge-region proline residues in domain, orientation. The current structure contributes to the understanding of, domain swapping in cyanovirins, permitting rational design of, domain-swapped CV-N mutants.
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<StructureSection load='1lom' size='340' side='right'caption='[[1lom]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LOM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lom OCA], [https://pdbe.org/1lom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lom RCSB], [https://www.ebi.ac.uk/pdbsum/1lom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lom ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lo/1lom_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lom ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cyanovirin-N (CV-N) is a potent 11 kDa HIV-inactivating protein that binds with high affinity to the HIV surface envelope protein gp120. A double mutant P51S/S52P of CV-N was engineered by swapping two critical hinge-region residues Pro51 and Ser52. This mutant has biochemical and biophysical characteristics equivalent to the wild-type CV-N and its structure resembles that of wild-type CV-N. However, the mutant shows a different orientation in the hinge region that connects two domains of the protein. The observation that this double mutant crystallizes under a wide variety of conditions challenges some of the current hypotheses on domain swapping and on the role of hinge-region proline residues in domain orientation. The current structure contributes to the understanding of domain swapping in cyanovirins, permitting rational design of domain-swapped CV-N mutants.
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==About this Structure==
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Domain-swapped structure of a mutant of cyanovirin-N.,Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A Biochem Biophys Res Commun. 2002 May 31;294(1):184-90. PMID:12054761<ref>PMID:12054761</ref>
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1LOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with SO4 and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LOM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Domain-swapped structure of a mutant of cyanovirin-N., Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A, Biochem Biophys Res Commun. 2002 May 31;294(1):184-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12054761 12054761]
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</div>
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<div class="pdbe-citations 1lom" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Nostoc ellipsosporum]]
[[Category: Nostoc ellipsosporum]]
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[[Category: Single protein]]
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[[Category: Botos I]]
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[[Category: Botos, I.]]
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[[Category: Boyd MR]]
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[[Category: Boyd, M.R.]]
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[[Category: Cartner LK]]
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[[Category: Cartner, L.K.]]
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[[Category: Mori T]]
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[[Category: Mori, T.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: CA]]
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[[Category: SO4]]
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[[Category: cyanovirin-n]]
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[[Category: domain-swapping]]
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[[Category: gp120]]
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[[Category: hiv-inactivating]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:17:24 2007''
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Current revision

CYANOVIRIN-N DOUBLE MUTANT P51S S52P

PDB ID 1lom

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