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1nam

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(New page: 200px<br /><applet load="1nam" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nam, resolution 2.70&Aring;" /> '''MURINE ALLOREACTIVE ...)
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[[Image:1nam.gif|left|200px]]<br /><applet load="1nam" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nam, resolution 2.70&Aring;" />
 
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'''MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX'''<br />
 
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==Overview==
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==MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX==
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T cell receptor (TCR) binding degeneracy lies at the heart of several, physiological and pathological phenomena, yet its structural basis is, poorly understood. We determined the crystal structure of a complex, involving the BM3.3 TCR and an octapeptide (VSV8) bound to the H-2K(b), major histocompatibility complex molecule at a 2.7 A resolution, and, compared it with the BM3.3 TCR bound to the H-2K(b) molecule loaded with a, peptide that has no primary sequence identity with VSV8. Comparison of, these structures showed that the BM3.3 TCR complementarity-determining, region (CDR) 3alpha could undergo rearrangements to adapt to structurally, different peptide residues. Therefore, CDR3 loop flexibility helps explain, TCR binding cross-reactivity.
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<StructureSection load='1nam' size='340' side='right'caption='[[1nam]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nam]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus_strain_Glasgow Vesicular stomatitis Indiana virus strain Glasgow]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NAM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nam OCA], [https://pdbe.org/1nam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nam RCSB], [https://www.ebi.ac.uk/pdbsum/1nam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nam ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1nam_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nam ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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T cell receptor (TCR) binding degeneracy lies at the heart of several physiological and pathological phenomena, yet its structural basis is poorly understood. We determined the crystal structure of a complex involving the BM3.3 TCR and an octapeptide (VSV8) bound to the H-2K(b) major histocompatibility complex molecule at a 2.7 A resolution, and compared it with the BM3.3 TCR bound to the H-2K(b) molecule loaded with a peptide that has no primary sequence identity with VSV8. Comparison of these structures showed that the BM3.3 TCR complementarity-determining region (CDR) 3alpha could undergo rearrangements to adapt to structurally different peptide residues. Therefore, CDR3 loop flexibility helps explain TCR binding cross-reactivity.
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==About this Structure==
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CDR3 loop flexibility contributes to the degeneracy of TCR recognition.,Reiser JB, Darnault C, Gregoire C, Mosser T, Mazza G, Kearney A, van der Merwe PA, Fontecilla-Camps JC, Housset D, Malissen B Nat Immunol. 2003 Mar;4(3):241-7. Epub 2003 Feb 3. PMID:12563259<ref>PMID:12563259</ref>
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1NAM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NAM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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CDR3 loop flexibility contributes to the degeneracy of TCR recognition., Reiser JB, Darnault C, Gregoire C, Mosser T, Mazza G, Kearney A, van der Merwe PA, Fontecilla-Camps JC, Housset D, Malissen B, Nat Immunol. 2003 Mar;4(3):241-7. Epub 2003 Feb 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12563259 12563259]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1nam" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Darnault, C.]]
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[[Category: Fontecilla-Camps, J.C.]]
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[[Category: Gregoire, C.]]
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[[Category: Housset, D.]]
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[[Category: Kearnay, A.]]
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[[Category: Malissen, B.]]
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[[Category: Mazza, G.]]
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[[Category: Merwe, P.A.van.der.]]
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[[Category: Mosser, T.]]
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[[Category: Reiser, J.B.]]
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[[Category: alloreactivity]]
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[[Category: class i mhc]]
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[[Category: crossreactivity]]
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[[Category: h-2kb]]
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[[Category: t cell receptor]]
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[[Category: tcr-pmhc complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:05:58 2007''
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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*[[MHC 3D structures|MHC 3D structures]]
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*[[MHC I 3D structures|MHC I 3D structures]]
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*[[T-cell receptor 3D structures|T-cell receptor 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Vesicular stomatitis Indiana virus strain Glasgow]]
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[[Category: Darnault C]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Gregoire C]]
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[[Category: Housset D]]
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[[Category: Kearnay A]]
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[[Category: Malissen B]]
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[[Category: Mazza G]]
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[[Category: Mosser T]]
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[[Category: Reiser J-B]]
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[[Category: Van der Merwe PA]]

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MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX

PDB ID 1nam

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