1njg

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(New page: 200px<br /><applet load="1njg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1njg, resolution 2.20&Aring;" /> '''Nucleotide-free form...)
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[[Image:1njg.gif|left|200px]]<br /><applet load="1njg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1njg, resolution 2.20&Aring;" />
 
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'''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit'''<br />
 
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==Overview==
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==Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit==
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Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes., The structure of the E. coli clamp loader in a nucleotide-free state has, been determined previously. We now report crystal structures of a, truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the, E. coli clamp loader, in nucleotide-free and bound forms. The gamma, subunit adopts a defined conformation when empty, in which the nucleotide, binding site is blocked. The binding of either ATPgammaS or ADP, which are, shown to bind with equal affinity to gamma(1-243), induces a change in the, relative orientation of the two domains such that nucleotides can be, accommodated. This change would break one of the gamma:gamma interfaces, seen in the empty clamp loader complex, and may represent one step in the, activation process.
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<StructureSection load='1njg' size='340' side='right'caption='[[1njg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1njg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1njg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njg OCA], [https://pdbe.org/1njg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1njg RCSB], [https://www.ebi.ac.uk/pdbsum/1njg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1njg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPO3X_ECOLI DPO3X_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. Isoform tau: serves as a scaffold to help in the dimerization of the core complex. Isoform gamma: seems to interact with the delta subunit. to transfer the beta subunit on the DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/1njg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1njg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
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==About this Structure==
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Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit.,Podobnik M, Weitze TF, O'Donnell M, Kuriyan J Structure. 2003 Mar;11(3):253-63. PMID:12623013<ref>PMID:12623013</ref>
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1NJG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12623013 12623013]
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</div>
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[[Category: DNA-directed DNA polymerase]]
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<div class="pdbe-citations 1njg" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Donnell, M.O.]]
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[[Category: Kuriyan, J.]]
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[[Category: Podobnik, M.]]
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[[Category: Weitze, T.F.]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: aaa+ atpase domains]]
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[[Category: rossman-like fold]]
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[[Category: sensor 1]]
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[[Category: sensor 2]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:19:01 2007''
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==See Also==
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Kuriyan J]]
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[[Category: O'Donnell M]]
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[[Category: Podobnik M]]
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[[Category: Weitze TF]]

Current revision

Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit

PDB ID 1njg

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