1qw0

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[[Image:1qw0.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Haemophilus influenzae N175L mutant Holo Ferric ion-Binding Protein A==
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|PDB= 1qw0 |SIZE=350|CAPTION= <scene name='initialview01'>1qw0</scene>, resolution 1.90&Aring;
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<StructureSection load='1qw0' size='340' side='right'caption='[[1qw0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1qw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QW0 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= FBPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qw0 OCA], [https://pdbe.org/1qw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qw0 RCSB], [https://www.ebi.ac.uk/pdbsum/1qw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qw0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FBPA_HAEIN FBPA_HAEIN] Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. This protein specifically binds Fe(3+) and is involved in its transmembrane transport (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qw0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The periplasmic iron binding protein plays an essential role in the iron uptake pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and Neisseriaceae families and is critical for survival of these pathogens within the host. In this study, we report the crystal structures of two mutant forms of ferric ion-binding protein A (FbpA) from Haemophilus influenzae with bound multinuclear oxo-metal clusters. Crystals of site-directed mutants in the metal or anion binding ligands contain protein in the open conformation, and two mutant FbpAs, H9A and N175L, contain different cluster arrangements in the iron-binding pocket. The iron clusters are anchored by binding to the two tyrosine ligands (Tyr195 and Tyr196) positioned at the vertex of the iron-binding pocket but are not coordinated by the other metal binding ligands. Our results suggest that the metal clusters may have formed in situ, suggesting that the mutant FbpAs may serve as a simple model for protein-mediated mineralization.
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'''Crystal Structure of Haemophilus influenzae N175L mutant Holo Ferric ion-Binding Protein A'''
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Presence of ferric hydroxide clusters in mutants of Haemophilus influenzae ferric ion-binding protein A.,Shouldice SR, Skene RJ, Dougan DR, McRee DE, Tari LW, Schryvers AB Biochemistry. 2003 Oct 21;42(41):11908-14. PMID:14556621<ref>PMID:14556621</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1qw0" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The periplasmic iron binding protein plays an essential role in the iron uptake pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and Neisseriaceae families and is critical for survival of these pathogens within the host. In this study, we report the crystal structures of two mutant forms of ferric ion-binding protein A (FbpA) from Haemophilus influenzae with bound multinuclear oxo-metal clusters. Crystals of site-directed mutants in the metal or anion binding ligands contain protein in the open conformation, and two mutant FbpAs, H9A and N175L, contain different cluster arrangements in the iron-binding pocket. The iron clusters are anchored by binding to the two tyrosine ligands (Tyr195 and Tyr196) positioned at the vertex of the iron-binding pocket but are not coordinated by the other metal binding ligands. Our results suggest that the metal clusters may have formed in situ, suggesting that the mutant FbpAs may serve as a simple model for protein-mediated mineralization.
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*[[Ferric-binding protein|Ferric-binding protein]]
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== References ==
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==About this Structure==
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<references/>
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1QW0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QW0 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Presence of ferric hydroxide clusters in mutants of Haemophilus influenzae ferric ion-binding protein A., Shouldice SR, Skene RJ, Dougan DR, McRee DE, Tari LW, Schryvers AB, Biochemistry. 2003 Oct 21;42(41):11908-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14556621 14556621]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dougan, D R.]]
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[[Category: Dougan DR]]
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[[Category: McRee, D E.]]
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[[Category: McRee DE]]
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[[Category: Schryvers, A B.]]
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[[Category: Schryvers AB]]
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[[Category: Shouldice, S R.]]
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[[Category: Shouldice SR]]
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[[Category: Skene, R J.]]
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[[Category: Skene RJ]]
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[[Category: Tari, L W.]]
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[[Category: Tari LW]]
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[[Category: FE]]
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[[Category: PO4]]
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[[Category: metal binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:43:28 2008''
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Current revision

Crystal Structure of Haemophilus influenzae N175L mutant Holo Ferric ion-Binding Protein A

PDB ID 1qw0

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