1zij

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(New page: 200px<br /><applet load="1zij" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zij, resolution 2.00&Aring;" /> '''GCN4-LEUCINE ZIPPER ...)
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[[Image:1zij.gif|left|200px]]<br /><applet load="1zij" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zij, resolution 2.00&Aring;" />
 
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'''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE'''<br />
 
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==Overview==
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==GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE==
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Each protein sequence generally adopts a single native fold, but the, sequence features that confer structural uniqueness are not well, understood. To define the basis for structural heterogeneity, we, determined the high resolution X-ray crystal structures of a single GCN4, leucine-zipper mutant (Asn 16 to aminobutyric acid) in both dimeric and, trimeric coiled-coil conformations. The mutant sequence is accommodated in, two distinct structures by forming similarly-shaped packing surfaces with, different sets of atoms. The trimer structure, in comparison to a, previously-characterized trimeric mutant with substitutions in eight core, residues, shows that the twist of individual helices and the helix-helix, crossing angles can vary significantly to produce the most favoured, packing arrangement.
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<StructureSection load='1zij' size='340' side='right'caption='[[1zij]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zij]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zij OCA], [https://pdbe.org/1zij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zij RCSB], [https://www.ebi.ac.uk/pdbsum/1zij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zij ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Each protein sequence generally adopts a single native fold, but the sequence features that confer structural uniqueness are not well understood. To define the basis for structural heterogeneity, we determined the high resolution X-ray crystal structures of a single GCN4 leucine-zipper mutant (Asn 16 to aminobutyric acid) in both dimeric and trimeric coiled-coil conformations. The mutant sequence is accommodated in two distinct structures by forming similarly-shaped packing surfaces with different sets of atoms. The trimer structure, in comparison to a previously-characterized trimeric mutant with substitutions in eight core residues, shows that the twist of individual helices and the helix-helix crossing angles can vary significantly to produce the most favoured packing arrangement.
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==About this Structure==
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Crystal structures of a single coiled-coil peptide in two oligomeric states reveal the basis for structural polymorphism.,Gonzalez L Jr, Brown RA, Richardson D, Alber T Nat Struct Biol. 1996 Dec;3(12):1002-9. PMID:8946853<ref>PMID:8946853</ref>
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1ZIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of a single coiled-coil peptide in two oligomeric states reveal the basis for structural polymorphism., Gonzalez L Jr, Brown RA, Richardson D, Alber T, Nat Struct Biol. 1996 Dec;3(12):1002-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8946853 8946853]
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</div>
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[[Category: Saccharomyces cerevisiae]]
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<div class="pdbe-citations 1zij" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Alber, T.]]
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[[Category: Brown, R.A.]]
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[[Category: Junior, L.Gonzalez.]]
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[[Category: Richardson, D.]]
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[[Category: ACE]]
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[[Category: activator]]
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[[Category: amino-acid biosynthesis]]
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[[Category: coiled coil]]
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[[Category: dna-binding]]
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[[Category: leucine zipper]]
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[[Category: nuclear protein]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:28:34 2007''
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==See Also==
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Alber T]]
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[[Category: Brown RA]]
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[[Category: Gonzalez Junior L]]
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[[Category: Richardson D]]

Current revision

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16ABA IN THE TRIMERIC STATE

PDB ID 1zij

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