This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2xg9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:39, 16 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2xg9.png|left|200px]]
 
-
{{STRUCTURE_2xg9| PDB=2xg9 | SCENE= }}
+
==Crystal structure of Barley Beta-Amylase complexed with 4-O-alpha-D- glucopyranosylmoranoline==
 +
<StructureSection load='2xg9' size='340' side='right'caption='[[2xg9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2xg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XG9 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xg9 OCA], [https://pdbe.org/2xg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2xg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xg9 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/AMYB_HORVU AMYB_HORVU]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major beta-amylase of the grain endosperm (BMY1). The molecular basis of inhibitor action was defined through high resolution X-ray crystallography studies of unliganded barley beta-amylase, as well as its complexes with glycone site binder disaccharide iminosugar G1M, irreversible inhibitors alpha-epoxypropyl and alpha-epoxybutyl glucosides, which target the enzyme's catalytic residues, and the aglycone site binders acarbose and alpha-cyclodextrin.
-
===CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH 4-O-ALPHA-D-GLUCOPYRANOSYLMORANOLINE===
+
Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase.,Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA Mol Biosyst. 2010 Nov 18. PMID:21085740<ref>PMID:21085740</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
[[2xg9]] is a 1 chain structure of [[Alpha-Amylase]] with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XG9 OCA].
+
<div class="pdbe-citations 2xg9" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
-
*[[Alpha-Amylase|Alpha-Amylase]]
+
*[[Amylase 3D structures|Amylase 3D structures]]
-
[[Category: Beta-amylase]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
-
[[Category: Denyer, K.]]
+
[[Category: Large Structures]]
-
[[Category: Field, R A.]]
+
[[Category: Denyer K]]
-
[[Category: Lawson, D M.]]
+
[[Category: Field RA]]
-
[[Category: Naldrett, M J.]]
+
[[Category: Lawson DM]]
-
[[Category: Rejzek, M.]]
+
[[Category: Naldrett MJ]]
-
[[Category: Smith, A M.]]
+
[[Category: Rejzek M]]
-
[[Category: Southard, A M.]]
+
[[Category: Smith AM]]
-
[[Category: Stanley, D.]]
+
[[Category: Southard AM]]
-
[[Category: Stevenson, C E.M.]]
+
[[Category: Stanley D]]
-
[[Category: Carbohydrate metabolism]]
+
[[Category: Stevenson CEM]]
-
[[Category: Germination]]
+
-
[[Category: Glycosidase]]
+
-
[[Category: Glycosyl hydrolase family 14]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Starch degradation]]
+

Current revision

Crystal structure of Barley Beta-Amylase complexed with 4-O-alpha-D- glucopyranosylmoranoline

PDB ID 2xg9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xg9"
Personal tools