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2xg9

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[[Image:2xg9.png|left|200px]]
 
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{{STRUCTURE_2xg9| PDB=2xg9 | SCENE= }}
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==Crystal structure of Barley Beta-Amylase complexed with 4-O-alpha-D- glucopyranosylmoranoline==
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<StructureSection load='2xg9' size='340' side='right'caption='[[2xg9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XG9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NOJ:1-DEOXYNOJIRIMYCIN'>NOJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xg9 OCA], [https://pdbe.org/2xg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2xg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xg9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMYB_HORVU AMYB_HORVU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major beta-amylase of the grain endosperm (BMY1). The molecular basis of inhibitor action was defined through high resolution X-ray crystallography studies of unliganded barley beta-amylase, as well as its complexes with glycone site binder disaccharide iminosugar G1M, irreversible inhibitors alpha-epoxypropyl and alpha-epoxybutyl glucosides, which target the enzyme's catalytic residues, and the aglycone site binders acarbose and alpha-cyclodextrin.
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===CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH 4-O-ALPHA-D-GLUCOPYRANOSYLMORANOLINE===
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Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase.,Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA Mol Biosyst. 2010 Nov 18. PMID:21085740<ref>PMID:21085740</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2xg9]] is a 1 chain structure of [[Alpha-Amylase]] with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XG9 OCA].
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<div class="pdbe-citations 2xg9" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Alpha-Amylase|Alpha-Amylase]]
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*[[Amylase 3D structures|Amylase 3D structures]]
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[[Category: Beta-amylase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Denyer, K.]]
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[[Category: Large Structures]]
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[[Category: Field, R A.]]
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[[Category: Denyer K]]
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[[Category: Lawson, D M.]]
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[[Category: Field RA]]
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[[Category: Naldrett, M J.]]
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[[Category: Lawson DM]]
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[[Category: Rejzek, M.]]
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[[Category: Naldrett MJ]]
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[[Category: Smith, A M.]]
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[[Category: Rejzek M]]
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[[Category: Southard, A M.]]
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[[Category: Smith AM]]
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[[Category: Stanley, D.]]
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[[Category: Southard AM]]
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[[Category: Stevenson, C E.M.]]
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[[Category: Stanley D]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Stevenson CEM]]
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[[Category: Germination]]
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[[Category: Glycosidase]]
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[[Category: Glycosyl hydrolase family 14]]
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[[Category: Hydrolase]]
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[[Category: Starch degradation]]
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Current revision

Crystal structure of Barley Beta-Amylase complexed with 4-O-alpha-D- glucopyranosylmoranoline

PDB ID 2xg9

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