This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5hwp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5hwp is ON HOLD Authors: Bock, T., Kasten, J., Blankenfeldt, W. Description: Category: Unreleased Structures Category: Bock, T [[Category:...) |
|||
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==MvaS with acetylated Cys115 in complex with coenzyme A== | |
| + | <StructureSection load='5hwp' size='340' side='right'caption='[[5hwp]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hwp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HWP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwp OCA], [https://pdbe.org/5hwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hwp RCSB], [https://www.ebi.ac.uk/pdbsum/5hwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q1D4I1_MYXXD Q1D4I1_MYXXD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A critical step in bacterial isoprenoid production is the synthesis of 3-hydroxy-3-methylglutaryl coenzyme A catalyzed by HMG-CoA synthase (HMGCS). In myxobacteria, this enzyme is also involved in a recently discovered acetyl-CoA-dependent isovaleryl-CoA biosynthesis pathway. Here we present crystal structures of MvaS, the HMGCS from Myxococcus xanthus, in complex with coenzyme A and acetylated active site Cys115, with the second substrate acetoacetyl-CoA and with the product 3-hydroxy-3-methylglutaryl-CoA. We show that MvaS uses the common HMGCS enzymatic mechanism and provide evidence that dimerization plays a role in the formation and stability of the active site. Overall, MvaS shows typical features of the eukaryotic HMGCS and exhibits differences to homologs from Gram-positive bacteria. This study provides insights into myxobacterial alternative isovaleryl coenzyme A biosynthesis and thereby extends the toolbox for the biotechnological production of renewable fuel and chemicals. | ||
| - | + | Crystal structure of the HMG-CoA synthase MvaS from the Gram-negative bacterium Myxococcus xanthus.,Bock T, Kasten J, Muller R, Blankenfeldt W Chembiochem. 2016 Apr 28. doi: 10.1002/cbic.201600070. PMID:27124816<ref>PMID:27124816</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5hwp" style="background-color:#fffaf0;"></div> |
| - | [[Category: Blankenfeldt | + | == References == |
| - | [[Category: Kasten | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Myxococcus xanthus DK 1622]] | ||
| + | [[Category: Blankenfeldt W]] | ||
| + | [[Category: Bock T]] | ||
| + | [[Category: Kasten J]] | ||
Current revision
MvaS with acetylated Cys115 in complex with coenzyme A
| |||||||||||
