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1r0o

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(New page: 200px<br /><applet load="1r0o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r0o, resolution 2.24&Aring;" /> '''Crystal Structure of...)
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[[Image:1r0o.gif|left|200px]]<br /><applet load="1r0o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r0o, resolution 2.24&Aring;" />
 
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'''Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex'''<br />
 
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==Overview==
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==Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex==
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Ecdysteroids initiate molting and metamorphosis in insects via a, heterodimeric receptor consisting of the ecdysone receptor (EcR) and, ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates, transcription through highly degenerate pseudo-palindromic response, elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp, (IR-1). The requirement for a heterodimeric arrangement of EcR-USP, subunits to bind to a symmetric DNA is unusual within the nuclear receptor, superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding, domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP, use similar surfaces, and rely on the deformed minor groove of the DNA to, establish protein-protein contacts. As retinoid X receptor (RXR) is the, mammalian homolog of USP, we also solved the 2.60 A crystal structure of, the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and, DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence, alignments indicate that the EcR-RXR heterodimer is an important model for, understanding how the FXR-RXR heterodimer binds to IR-1 sites.
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<StructureSection load='1r0o' size='340' side='right'caption='[[1r0o]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R0O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0o OCA], [https://pdbe.org/1r0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r0o RCSB], [https://www.ebi.ac.uk/pdbsum/1r0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r0o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/USP_DROME USP_DROME] Receptor for ecdysone. May be an important modulator of insect metamorphosis. Plays an important part in embryonic and post-embryonic development. Binds to ecdysone response elements (ECRES) such as in the promoter region of s15 chorion gene.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/1r0o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r0o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.
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==About this Structure==
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Structure of the heterodimeric ecdysone receptor DNA-binding complex.,Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F EMBO J. 2003 Nov 3;22(21):5827-40. PMID:14592980<ref>PMID:14592980</ref>
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1R0O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R0O OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14592980 14592980]
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</div>
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<div class="pdbe-citations 1r0o" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Devarakonda, S.]]
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[[Category: Devarakonda S]]
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[[Category: Harp, J.M.]]
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[[Category: Harp JM]]
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[[Category: Kim, Y.]]
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[[Category: Kim Y]]
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[[Category: Ozyhar, A.]]
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[[Category: Ozyhar A]]
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[[Category: Rastinejad, F.]]
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[[Category: Rastinejad F]]
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[[Category: ZN]]
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[[Category: dna binding domain]]
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[[Category: ecdsyone receptor]]
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[[Category: nuclear receptor]]
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[[Category: ultraspiracle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:10:10 2007''
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Current revision

Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex

PDB ID 1r0o

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