1ra4

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(New page: 200px<br /><applet load="1ra4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ra4, resolution 1.86&Aring;" /> '''Crystal structure of...)
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[[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ra4, resolution 1.86&Aring;" />
 
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'''Crystal structure of the Methanococcus jannaschii L7Ae protein'''<br />
 
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==Overview==
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==Crystal structure of the Methanococcus jannaschii L7Ae protein==
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Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein, that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D, sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has, been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary, conservation, functional characteristics, and structural details have been, analyzed. Comparison of the L7Ae structure to those of a number of related, proteins with diverse functions has revealed significant structural, homology which suggests that this protein fold is an ancient RNA-binding, motif. Notably, the free M. jannaschii L7Ae structure is essentially, identical to that with RNA bound, suggesting that RNA binding occurs, through an induced-fit interaction. Circular dichroism experiments show, that box C/D and C'/D' RNA motifs undergo conformational changes when, magnesium or the L7Ae protein is added, corroborating the induced-fit, model for L7Ae-box C/D RNA interactions.
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<StructureSection load='1ra4' size='340' side='right'caption='[[1ra4]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ra4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RA4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ra4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ra4 OCA], [https://pdbe.org/1ra4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ra4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ra4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ra4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL7A_METJA RL7A_METJA] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/1ra4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ra4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.
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==About this Structure==
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The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs.,Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd Biochemistry. 2005 Jul 19;44(28):9657-72. PMID:16008351<ref>PMID:16008351</ref>
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1RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs., Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd, Biochemistry. 2005 Jul 19;44(28):9657-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16008351 16008351]
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</div>
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[[Category: Methanocaldococcus jannaschii]]
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<div class="pdbe-citations 1ra4" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Brown, B.A.]]
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[[Category: Maxwell, E.S.]]
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[[Category: Suryadi, J.]]
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[[Category: Tran, E.J.]]
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[[Category: alpha-beta-alpha sandwich fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:23:17 2007''
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==See Also==
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*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Methanocaldococcus jannaschii]]
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[[Category: Brown BA]]
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[[Category: Maxwell ES]]
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[[Category: Suryadi J]]
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[[Category: Tran EJ]]

Current revision

Crystal structure of the Methanococcus jannaschii L7Ae protein

PDB ID 1ra4

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