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1rjl

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(New page: 200px<br /> <applet load="1rjl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rjl, resolution 2.60&Aring;" /> '''Structure of the co...)
Current revision (06:05, 23 August 2023) (edit) (undo)
 
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[[Image:1rjl.gif|left|200px]]<br />
 
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<applet load="1rjl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rjl, resolution 2.60&Aring;" />
 
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'''Structure of the complex between OspB-CT and bactericidal Fab-H6831'''<br />
 
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==Overview==
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==Structure of the complex between OspB-CT and bactericidal Fab-H6831==
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Certain antibody Fab fragments directed against the C terminus of outer, surface protein B (OspB), a major lipoprotein of the Lyme disease, spirochete, Borrelia burgdorferi, have the unusual property of being, bactericidal even in the absence of complement. We report here x-ray, crystal structures of a C-terminal fragment of B. burgdorferi OspB, which, spans residues 152-296, alone at 2.0-A resolution, and in a complex with, the bactericidal Fab H6831 at 2.6-A resolution. The H6831 epitope is, topologically analogous to the LA-2 epitope of OspA and is centered around, OspB Lys-253, a residue essential for H6831 recognition. A beta-sheet, present in the free OspB fragment is either disordered or removed by, proteolysis in the H6831-bound complex. Other conformational changes, between free and H6831-bound structures are minor and appear to be related, to this loss. In both crystal structures, OspB C-terminal fragments form, artificial dimers connected by intermolecular beta-sheets. OspB structure, stability, and possible mechanisms of killing by H6831 and other, bactericidal Fabs are discussed in light of the structural data.
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<StructureSection load='1rjl' size='340' side='right'caption='[[1rjl]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rjl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rjl OCA], [https://pdbe.org/1rjl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rjl RCSB], [https://www.ebi.ac.uk/pdbsum/1rjl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rjl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OSPB_BORBU OSPB_BORBU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/1rjl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rjl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Certain antibody Fab fragments directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi, have the unusual property of being bactericidal even in the absence of complement. We report here x-ray crystal structures of a C-terminal fragment of B. burgdorferi OspB, which spans residues 152-296, alone at 2.0-A resolution, and in a complex with the bactericidal Fab H6831 at 2.6-A resolution. The H6831 epitope is topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for H6831 recognition. A beta-sheet present in the free OspB fragment is either disordered or removed by proteolysis in the H6831-bound complex. Other conformational changes between free and H6831-bound structures are minor and appear to be related to this loss. In both crystal structures, OspB C-terminal fragments form artificial dimers connected by intermolecular beta-sheets. OspB structure, stability, and possible mechanisms of killing by H6831 and other bactericidal Fabs are discussed in light of the structural data.
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==About this Structure==
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Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target.,Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683<ref>PMID:15713683</ref>
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1RJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RJL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15713683 15713683]
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</div>
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[[Category: Borrelia burgdorferi]]
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<div class="pdbe-citations 1rjl" style="background-color:#fffaf0;"></div>
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[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Barbour, A.G.]]
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[[Category: Becker, M.]]
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[[Category: Bunikis, J.]]
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[[Category: Dunn, J.J.]]
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[[Category: Lade, B.D.]]
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[[Category: Lawson, C.L.]]
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[[Category: antibody-protein complex]]
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[[Category: beta sheet]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:41:26 2007''
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Outer surface protein|Outer surface protein]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Borreliella burgdorferi]]
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Barbour AG]]
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[[Category: Becker M]]
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[[Category: Bunikis J]]
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[[Category: Dunn JJ]]
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[[Category: Lade BD]]
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[[Category: Lawson CL]]

Current revision

Structure of the complex between OspB-CT and bactericidal Fab-H6831

PDB ID 1rjl

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