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| | <StructureSection load='1rkv' size='340' side='right'caption='[[1rkv]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='1rkv' size='340' side='right'caption='[[1rkv]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1rkv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RKV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1rkv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RKV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rku|1rku]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ThrH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkv OCA], [https://pdbe.org/1rkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rkv RCSB], [https://www.ebi.ac.uk/pdbsum/1rkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rkv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkv OCA], [http://pdbe.org/1rkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rkv RCSB], [http://www.ebi.ac.uk/pdbsum/1rkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rkv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/THRH_PSEAE THRH_PSEAE]] Phosphoserine phosphatase that mediates dephosphorylation of phosphoserine in the serine biosynthesis pathway. Also able to dephosphorylate other substrates such as phospho-L(or D)-threonine, with lower activity. Shows phosphoserine:homoserine phosphotransferase activity by transferring the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor.<ref>PMID:10220164</ref> <ref>PMID:14699121</ref> | + | [https://www.uniprot.org/uniprot/THRH_PSEAE THRH_PSEAE] Phosphoserine phosphatase that mediates dephosphorylation of phosphoserine in the serine biosynthesis pathway. Also able to dephosphorylate other substrates such as phospho-L(or D)-threonine, with lower activity. Shows phosphoserine:homoserine phosphotransferase activity by transferring the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor.<ref>PMID:10220164</ref> <ref>PMID:14699121</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Huynh, T]] | + | [[Category: Huynh T]] |
| - | [[Category: Karthikeyan, S]] | + | [[Category: Karthikeyan S]] |
| - | [[Category: Phillips, M A]] | + | [[Category: Phillips MA]] |
| - | [[Category: Singh, S K]] | + | [[Category: Singh SK]] |
| - | [[Category: Subramanian, K]] | + | [[Category: Subramanian K]] |
| - | [[Category: Yang, K]] | + | [[Category: Yang K]] |
| - | [[Category: Zhang, H]] | + | [[Category: Zhang H]] |
| - | [[Category: Zhang, X]] | + | [[Category: Zhang X]] |
| - | [[Category: Phosphoserine phosphatase]]
| + | |
| - | [[Category: Phosphoserine phosphoryl donor]]
| + | |
| - | [[Category: Phosphoserine:homoserine phosphotransferase]]
| + | |
| - | [[Category: Thrh]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
THRH_PSEAE Phosphoserine phosphatase that mediates dephosphorylation of phosphoserine in the serine biosynthesis pathway. Also able to dephosphorylate other substrates such as phospho-L(or D)-threonine, with lower activity. Shows phosphoserine:homoserine phosphotransferase activity by transferring the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The thrH gene product of Pseudomonas aeruginosa has been shown to complement both homoserine kinase (thrB gene product) and phosphoserine phosphatase (serB gene product) activities in vivo. Sequence comparison has revealed that ThrH is related to phosphoserine phosphatases (PSP, EC 3.1.3.3) and belongs to the l-2-haloacid dehalogenase-like protein superfamily. We have solved the crystal structures of ThrH in the apoform and in complex with a bound product phosphate. The structure confirms an overall fold similar to that of PSP. Most of the catalytic residues of PSP are also conserved in ThrH, suggesting that similar catalytic mechanisms are used by both enzymes. Spectrophotometry-based in vitro assays show that ThrH is indeed a phosphoserine phosphatase with a K(m) of 0.207 mm and k(cat) of 13.4 min(-1), comparable with those of other PSPs. More interestingly, using high pressure liquid chromatography-based assays, we have demonstrated that ThrH is able to further transfer the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor, indicating that ThrH has a novel phosphoserine:homoserine phosphotransferase activity.
The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity.,Singh SK, Yang K, Karthikeyan S, Huynh T, Zhang X, Phillips MA, Zhang H J Biol Chem. 2004 Mar 26;279(13):13166-73. Epub 2003 Dec 29. PMID:14699121[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Patte JC, Clepet C, Bally M, Borne F, Mejean V, Foglino M. ThrH, a homoserine kinase isozyme with in vivo phosphoserine phosphatase activity in Pseudomonas aeruginosa. Microbiology. 1999 Apr;145 ( Pt 4):845-53. PMID:10220164
- ↑ Singh SK, Yang K, Karthikeyan S, Huynh T, Zhang X, Phillips MA, Zhang H. The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity. J Biol Chem. 2004 Mar 26;279(13):13166-73. Epub 2003 Dec 29. PMID:14699121 doi:http://dx.doi.org/10.1074/jbc.M311393200
- ↑ Singh SK, Yang K, Karthikeyan S, Huynh T, Zhang X, Phillips MA, Zhang H. The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity. J Biol Chem. 2004 Mar 26;279(13):13166-73. Epub 2003 Dec 29. PMID:14699121 doi:http://dx.doi.org/10.1074/jbc.M311393200
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