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1rxz

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(New page: 200px<br /><applet load="1rxz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rxz, resolution 2.0&Aring;" /> '''C-terminal region of ...)
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[[Image:1rxz.gif|left|200px]]<br /><applet load="1rxz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rxz, resolution 2.0&Aring;" />
 
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'''C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA'''<br />
 
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==Overview==
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==C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA==
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Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell, nuclear antigen (PCNA) are central to DNA replication and repair. To, clarify the molecular basis of FEN-1 specificity and PCNA activation, we, report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational, results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks, the DNA, and promotes conformational closing of a flexible helical clamp, to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal, regions in FEN-1 and PCNA creates an intermolecular beta sheet interface, that directly links adjacent PCNA and DNA binding regions of FEN-1 and, suggests how PCNA stimulates FEN-1 activity. The DNA and protein, conformational changes, composite complex structures, FRET, and mutational, results support enzyme-PCNA alignments and a kinked DNA pivot point that, appear suitable to coordinate rotary handoffs of kinked DNA intermediates, among enzymes localized by the three PCNA binding sites.
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<StructureSection load='1rxz' size='340' side='right'caption='[[1rxz]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rxz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] and [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RXZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rxz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rxz OCA], [https://pdbe.org/1rxz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rxz RCSB], [https://www.ebi.ac.uk/pdbsum/1rxz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rxz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCNA_ARCFU PCNA_ARCFU] Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rx/1rxz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rxz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
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==About this Structure==
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Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair.,Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA Cell. 2004 Jan 9;116(1):39-50. PMID:14718165<ref>PMID:14718165</ref>
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1RXZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RXZ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14718165 14718165]
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</div>
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[[Category: Archaeoglobus fulgidus]]
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<div class="pdbe-citations 1rxz" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Chapados, B.R.]]
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[[Category: Han, S.]]
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[[Category: Hosfield, D.J.]]
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[[Category: Qiu, J.]]
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[[Category: Shen, B.]]
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[[Category: Tainer, J.A.]]
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[[Category: Yelent, B.]]
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[[Category: beta-zipper]]
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[[Category: dna repair]]
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[[Category: dna replication]]
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[[Category: interdomain connecting loop]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:56:46 2007''
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Archaeoglobus fulgidus]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Large Structures]]
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[[Category: Chapados BR]]
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[[Category: Han S]]
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[[Category: Hosfield DJ]]
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[[Category: Qiu J]]
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[[Category: Shen B]]
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[[Category: Tainer JA]]
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[[Category: Yelent B]]

Current revision

C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA

PDB ID 1rxz

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