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1s8n

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[[Image:1s8n.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Rv1626 from Mycobacterium tuberculosis==
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|PDB= 1s8n |SIZE=350|CAPTION= <scene name='initialview01'>1s8n</scene>, resolution 1.482&Aring;
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<StructureSection load='1s8n' size='340' side='right'caption='[[1s8n]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>
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<table><tr><td colspan='2'>[[1s8n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S8N FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.482&#8491;</td></tr>
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|GENE= rv1626 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam03861 ANTAR], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00156 REC], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG3707 AmiR]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8n OCA], [https://pdbe.org/1s8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s8n RCSB], [https://www.ebi.ac.uk/pdbsum/1s8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s8n ProSAT], [https://www.topsan.org/Proteins/XMTB/1s8n TOPSAN]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8n OCA], [http://www.ebi.ac.uk/pdbsum/1s8n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s8n RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PDTAR_MYCTU PDTAR_MYCTU] Member of the two-component regulatory system pdtaR/pdtaS.<ref>PMID:16026786</ref>
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== Evolutionary Conservation ==
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'''Crystal structure of Rv1626 from Mycobacterium tuberculosis'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s8/1s8n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s8n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii.
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii.
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==About this Structure==
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The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis.,Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA Structure. 2004 Sep;12(9):1595-605. PMID:15341725<ref>PMID:15341725</ref>
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1S8N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8N OCA].
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==Reference==
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The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis., Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA, Structure. 2004 Sep;12(9):1595-605. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341725 15341725]
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[[Category: Mycobacterium tuberculosis h37rv]]
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[[Category: Single protein]]
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[[Category: Feng, V.]]
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[[Category: Morth, J P.]]
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[[Category: Perry, L J.]]
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[[Category: Svergun, D I.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Tucker, P A.]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: rv1626]]
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[[Category: structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: transcriptional antiterminator]]
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[[Category: two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:52 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1s8n" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Feng V]]
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[[Category: Morth JP]]
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[[Category: Perry LJ]]
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[[Category: Svergun DI]]
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[[Category: Tucker PA]]

Current revision

Crystal structure of Rv1626 from Mycobacterium tuberculosis

PDB ID 1s8n

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