1sj9

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[[Image:1sj9.gif|left|200px]]
 
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==Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution==
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The line below this paragraph, containing "STRUCTURE_1sj9", creates the "Structure Box" on the page.
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<StructureSection load='1sj9' size='340' side='right'caption='[[1sj9]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sj9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJ9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1sj9| PDB=1sj9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sj9 OCA], [https://pdbe.org/1sj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sj9 RCSB], [https://www.ebi.ac.uk/pdbsum/1sj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sj9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UDP_SALTY UDP_SALTY] Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sj9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sj9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Uridine phosphorylase (UPh) catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose 1-phosphate and uracil in the pyrimidine-salvage pathway. The crystal structure of the Salmonella typhimurium uridine phosphorylase (StUPh) has been determined at 2.5 A resolution and refined to an R factor of 22.1% and an Rfree of 27.9%. The hexameric StUPh displays 32 point-group symmetry and utilizes both twofold and threefold non-crystallographic axes. A phosphate is bound at the active site and forms hydrogen bonds to Arg91, Arg30, Thr94 and Gly26 of one monomer and Arg48 of an adjacent monomer. The hexameric StUPh model reveals a close structural relationship to Escherichia coli uridine phosphorylase (EcUPh).
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'''Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution'''
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Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state.,Dontsova MV, Gabdoulkhakov AG, Molchan OK, Lashkov AA, Garber MB, Mironov AS, Zhukhlistova NE, Morgunova EY, Voelter W, Betzel C, Zhang Y, Ealick SE, Mikhailov AM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):337-40. Epub 2005 Mar 24. PMID:16511035<ref>PMID:16511035</ref>
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==Overview==
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Uridine phosphorylase (UPh) catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose 1-phosphate and uracil in the pyrimidine-salvage pathway. The crystal structure of the Salmonella typhimurium uridine phosphorylase (StUPh) has been determined at 2.5 A resolution and refined to an R factor of 22.1% and an Rfree of 27.9%. The hexameric StUPh displays 32 point-group symmetry and utilizes both twofold and threefold non-crystallographic axes. A phosphate is bound at the active site and forms hydrogen bonds to Arg91, Arg30, Thr94 and Gly26 of one monomer and Arg48 of an adjacent monomer. The hexameric StUPh model reveals a close structural relationship to Escherichia coli uridine phosphorylase (EcUPh).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1SJ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJ9 OCA].
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</div>
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<div class="pdbe-citations 1sj9" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state., Dontsova MV, Gabdoulkhakov AG, Molchan OK, Lashkov AA, Garber MB, Mironov AS, Zhukhlistova NE, Morgunova EY, Voelter W, Betzel C, Zhang Y, Ealick SE, Mikhailov AM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):337-40. Epub 2005 Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511035 16511035]
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*[[Uridine phosphorylase 3D structures|Uridine phosphorylase 3D structures]]
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[[Category: Salmonella typhimurium]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Uridine phosphorylase]]
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__TOC__
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[[Category: Betzel, C.]]
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</StructureSection>
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[[Category: Dontsova, M.]]
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[[Category: Large Structures]]
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[[Category: Ealick, S.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Gabdoulkhakov, A.]]
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[[Category: Betzel C]]
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[[Category: Garber, M.]]
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[[Category: Dontsova M]]
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[[Category: Mikhailov, A.]]
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[[Category: Ealick S]]
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[[Category: Morgunova, E.]]
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[[Category: Gabdoulkhakov A]]
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[[Category: Nikonov, S.]]
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[[Category: Garber M]]
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[[Category: Nucleoside phosphorylase]]
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[[Category: Mikhailov A]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:46:16 2008''
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[[Category: Morgunova E]]
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[[Category: Nikonov S]]

Current revision

Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution

PDB ID 1sj9

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