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1t1d

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[[Image:1t1d.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL==
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|PDB= 1t1d |SIZE=350|CAPTION= <scene name='initialview01'>1t1d</scene>, resolution 1.51&Aring;
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<StructureSection load='1t1d' size='340' side='right'caption='[[1t1d]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1t1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1D FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1d OCA], [https://pdbe.org/1t1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1d RCSB], [https://www.ebi.ac.uk/pdbsum/1t1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1d ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1d OCA], [http://www.ebi.ac.uk/pdbsum/1t1d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t1d RCSB]</span>
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[https://www.uniprot.org/uniprot/Q16968_APLCA Q16968_APLCA]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
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'''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''
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Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels.,Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290<ref>PMID:9886290</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1t1d" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1T1D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9886290 9886290]
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Kreusch, A.]]
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[[Category: Kreusch A]]
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[[Category: Pfaffinger, P J.]]
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[[Category: Pfaffinger PJ]]
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[[Category: Stevens, C F.]]
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[[Category: Stevens CF]]
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[[Category: aplysia kv1 1]]
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[[Category: potassium channel]]
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[[Category: proton transport]]
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[[Category: tetramerization domain]]
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[[Category: x-ray structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:49:50 2008''
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Current revision

CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL

PDB ID 1t1d

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