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1wbf

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WINGED BEAN LECTIN, SACCHARIDE FREE FORM

Structural highlights

1wbf is a 2 chain structure with sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LEC1_PSOTE Lectin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop.

Structure of basic winged-bean lectin and a comparison with its saccharide-bound form.,Manoj N, Srinivas VR, Suguna K Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Manoj N, Srinivas VR, Suguna K. Structure of basic winged-bean lectin and a comparison with its saccharide-bound form. Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wbf"

Categories: Large Structures | Psophocarpus tetragonolobus | Manoj N | Srinivas VR | Suguna K

@@ Line 3: / Line 3: @@
 <StructureSection load='1wbf' size='340' side='right'caption='[[1wbf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1wbf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WBF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1wbf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WBF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbf OCA], [http://pdbe.org/1wbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wbf RCSB], [http://www.ebi.ac.uk/pdbsum/1wbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbf ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbf OCA], [https://pdbe.org/1wbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wbf RCSB], [https://www.ebi.ac.uk/pdbsum/1wbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LEC1_PSOTE LEC1_PSOTE]] Lectin.
+[https://www.uniprot.org/uniprot/LEC1_PSOTE LEC1_PSOTE] Lectin.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 37: / Line 38: @@
 [[Category: Large Structures]]
 [[Category: Psophocarpus tetragonolobus]]
-[[Category: Manoj, N]]
+[[Category: Manoj N]]
-[[Category: Srinivas, V R]]
+[[Category: Srinivas VR]]
-[[Category: Suguna, K]]
+[[Category: Suguna K]]
-[[Category: Blood group specificity]]
-[[Category: Legume lectin]]
-[[Category: Protein crystallography]]
-[[Category: Saccharide free form]]
-[[Category: Sugar binding protein]]

1wbf

From Proteopedia

Current revision

WINGED BEAN LECTIN, SACCHARIDE FREE FORM

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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