1m8n
From Proteopedia
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'''Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501''' | '''Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501''' | ||
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[[Category: Jia, Z.]] | [[Category: Jia, Z.]] | ||
[[Category: Leinala, E K.]] | [[Category: Leinala, E K.]] | ||
| - | [[Category: | + | [[Category: Antifreeze protein]] |
| - | [[Category: | + | [[Category: Left-handed beta-helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:46:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:46, 2 May 2008
Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501
Overview
The insect spruce budworm (Choristoneura fumiferana)(Cf) produces a number of isoforms of its highly active antifreeze protein (CfAFP). Although most of the CfAFP isoforms are in the 9-kDa range, isoforms containing a 30- or 31-amino acid insertion have also been identified. Here we describe the functional and structural analysis of a selected long isoform, CfAFP-501. X-ray crystal structure determination reveals that the 31-amino acid insertion found in CfAFP-501 forms two additional loops within its highly regular beta-helical structure. This effectively extends the area of the two-dimensional Thr array and ice-binding surface of the protein. The larger isoform has 3 times the thermal hysteresis activity of the 9-kDa CfAFP-337. As well, a deletion of the 31-amino acid insertion within CfAFP-501 to form CfAFP-501-Delta-2-loop, results in a protein with reduced activity similar to the shorter CfAFP isoforms. Thus, the enhanced antifreeze activity of CfAFP-501 is directly correlated to the length of its beta-helical structure and hence the size of its ice-binding face.
About this Structure
1M8N is a Single protein structure of sequence from Choristoneura fumiferana. Full crystallographic information is available from OCA.
Reference
A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights., Leinala EK, Davies PL, Doucet D, Tyshenko MG, Walker VK, Jia Z, J Biol Chem. 2002 Sep 6;277(36):33349-52. Epub 2002 Jun 24. PMID:12105229 Page seeded by OCA on Sat May 3 00:46:02 2008
