1xns

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{{Seed}}
 
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[[Image:1xns.png|left|200px]]
 
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==Peptide trapped Holliday junction intermediate in Cre-loxP recombination==
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The line below this paragraph, containing "STRUCTURE_1xns", creates the "Structure Box" on the page.
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<StructureSection load='1xns' size='340' side='right'caption='[[1xns]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xns]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xns OCA], [https://pdbe.org/1xns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xns RCSB], [https://www.ebi.ac.uk/pdbsum/1xns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xns ProSAT]</span></td></tr>
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{{STRUCTURE_1xns| PDB=1xns | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xns_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xns ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cre recombinase is a prototypical member of the tyrosine recombinase family of site-specific recombinases. Members of this family of enzymes catalyze recombination between specific DNA sequences by cleaving and exchanging one pair of strands between the two substrate sites to form a 4-way Holliday junction (HJ) intermediate and then resolve the HJ intermediate to recombinant products by a second round of strand exchanges. Recently, hexapeptide inhibitors have been described that are capable of blocking the second strand exchange step in the tyrosine recombinase recombination pathway, leading to an accumulation of the HJ intermediate. These peptides are active in the lambda-integrase, Cre recombinase, and Flp recombinase systems and are potentially important tools for both in vitro mechanistic studies and as in vivo probes of cellular function. Here we present biochemical and crystallographic data that support a model where the peptide inhibitor binds in the center of the recombinase-bound DNA junction and interacts with solvent-exposed bases near the junction branch point. Peptide binding induces large conformational changes in the DNA strands of the HJ intermediate, which affect the active site geometries in the recombinase subunits.
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===Peptide trapped Holliday junction intermediate in Cre-loxP recombination===
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Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination.,Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD J Biol Chem. 2005 Mar 4;280(9):8290-9. Epub 2004 Dec 8. PMID:15591069<ref>PMID:15591069</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1xns" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15591069}}, adds the Publication Abstract to the page
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15591069 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15591069}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus P1]]
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1XNS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p1 Enterobacteria phage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNS OCA].
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[[Category: Large Structures]]
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[[Category: Ghosh K]]
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==Reference==
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[[Category: Guo F]]
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Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination., Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD, J Biol Chem. 2005 Mar 4;280(9):8290-9. Epub 2004 Dec 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15591069 15591069]
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[[Category: Lau CK]]
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[[Category: Enterobacteria phage p1]]
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[[Category: Segall AM]]
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[[Category: Single protein]]
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[[Category: Van Duyne GD]]
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[[Category: Duyne, G D.Van.]]
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[[Category: Ghosh, K.]]
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[[Category: Guo, F.]]
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[[Category: Lau, C K.]]
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[[Category: Segall, A M.]]
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[[Category: Cre recombinase]]
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[[Category: Holliday junction]]
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[[Category: Peptide inhibitor]]
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[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:10:42 2008''
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Current revision

Peptide trapped Holliday junction intermediate in Cre-loxP recombination

PDB ID 1xns

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