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1ys2

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Current revision

Burkholderia cepacia lipase complexed with hexylphosphonic acid (S) 2-methyl-3-phenylpropyl ester

Structural highlights

1ys2 is a 1 chain structure with sequence from Burkholderia cepacia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LIP_BURCE Catalyzes the hydrolysis of triglycerides.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Synthetic chemists often exploit the high enantioselectivity of lipases to prepare pure enantiomers of primary alcohols, but the molecular basis for this enantioselectivity is unknown. The crystal structures of two phosphonate transition-state analogs bound to Burkholderia cepacia lipase reveal this molecular basis for a typical primary alcohol: 2-methyl-3-phenyl-1-propanol. The enantiomeric alcohol moieties adopt surprisingly similar orientations, with only subtle differences that make it difficult to predict how to alter enantioselectivity. These structures, along with a survey of previous structures of enzyme bound enantiomers, reveal that binding of enantiomers does not involve an exchange of two substituent positions as most researchers assumed. Instead, the enantiomers adopt mirror-image packing, where three of the four substituents at the stereocenter lie in similar positions. The fourth substituent, hydrogen, points in opposite directions.

Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol.,Mezzetti A, Schrag JD, Cheong CS, Kazlauskas RJ Chem Biol. 2005 Apr;12(4):427-37. PMID:15850979^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mezzetti A, Schrag JD, Cheong CS, Kazlauskas RJ. Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol. Chem Biol. 2005 Apr;12(4):427-37. PMID:15850979 doi:10.1016/j.chembiol.2005.01.016

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ys2"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ys2' size='340' side='right'caption='[[1ys2]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ys2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YS2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ys2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YS2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2HS:HEXYLPHOSPHONIC+ACID+(S)-2-METHYL-3-PHENYLPROPYL+ESTER'>2HS</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lip|3lip]], [[1ys1|1ys1]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HS:HEXYLPHOSPHONIC+ACID+(S)-2-METHYL-3-PHENYLPROPYL+ESTER'>2HS</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ys2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ys2 OCA], [https://pdbe.org/1ys2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ys2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ys2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ys2 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ys2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ys2 OCA], [http://pdbe.org/1ys2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ys2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ys2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ys2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LIP_BURCE LIP_BURCE]] Catalyzes the hydrolysis of triglycerides.
+[https://www.uniprot.org/uniprot/LIP_BURCE LIP_BURCE] Catalyzes the hydrolysis of triglycerides.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 39: / Line 38: @@
 [[Category: Burkholderia cepacia]]
 [[Category: Large Structures]]
-[[Category: Triacylglycerol lipase]]
+[[Category: Cheong CS]]
-[[Category: Cheong, C S]]
+[[Category: Kazlauskas RJ]]
-[[Category: Kazlauskas, R J]]
+[[Category: Mezzetti A]]
-[[Category: Mezzetti, A]]
+[[Category: Schrag JD]]
-[[Category: Schrag, J D]]
-[[Category: Ca2++ ion]]
-[[Category: Cis peptide bond leu 234]]
-[[Category: Hydrolase]]

1ys2

From Proteopedia

Current revision

Burkholderia cepacia lipase complexed with hexylphosphonic acid (S) 2-methyl-3-phenylpropyl ester

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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