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1zp5

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[[Image:1zp5.gif|left|200px]]<br /><applet load="1zp5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zp5, resolution 1.80&Aring;" />
 
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'''Crystal structure of the complex between MMP-8 and a N-hydroxyurea inhibitor'''<br />
 
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==Overview==
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==Crystal structure of the complex between MMP-8 and a N-hydroxyurea inhibitor==
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<StructureSection load='1zp5' size='340' side='right'caption='[[1zp5]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZP5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2NI:N-{2-[(4-CYANO-1,1-BIPHENYL-4-YL)OXY]ETHYL}-N-HYDROXY-N-METHYLUREA'>2NI</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zp5 OCA], [https://pdbe.org/1zp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zp5 RCSB], [https://www.ebi.ac.uk/pdbsum/1zp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zp5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MMP8_HUMAN MMP8_HUMAN] Can degrade fibrillar type I, II, and III collagens.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/1zp5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zp5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The first crystallographic structure of an N-hydroxyurea inhibitor bound into the active site of a matrix metalloproteinase is reported. The ligand and three other analogues were prepared and studied as inhibitors of MMP-2, MMP-3, and MMP-8. The crystal structure of the complex with MMP-8 shows that the N-hydroxyurea, contrary to the analogous hydroxamate, binds the catalytic zinc ion in a monodentate rather than bidentate mode and with high out-of-plane distortion of the amide bonds.
The first crystallographic structure of an N-hydroxyurea inhibitor bound into the active site of a matrix metalloproteinase is reported. The ligand and three other analogues were prepared and studied as inhibitors of MMP-2, MMP-3, and MMP-8. The crystal structure of the complex with MMP-8 shows that the N-hydroxyurea, contrary to the analogous hydroxamate, binds the catalytic zinc ion in a monodentate rather than bidentate mode and with high out-of-plane distortion of the amide bonds.
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==About this Structure==
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N-Hydroxyurea as zinc binding group in matrix metalloproteinase inhibition: mode of binding in a complex with MMP-8.,Campestre C, Agamennone M, Tortorella P, Preziuso S, Biasone A, Gavuzzo E, Pochetti G, Mazza F, Hiller O, Tschesche H, Consalvi V, Gallina C Bioorg Med Chem Lett. 2006 Jan 1;16(1):20-4. Epub 2005 Oct 18. PMID:16242329<ref>PMID:16242329</ref>
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1ZP5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=2NI:'>2NI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Neutrophil_collagenase Neutrophil collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.34 3.4.24.34] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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N-Hydroxyurea as zinc binding group in matrix metalloproteinase inhibition: mode of binding in a complex with MMP-8., Campestre C, Agamennone M, Tortorella P, Preziuso S, Biasone A, Gavuzzo E, Pochetti G, Mazza F, Hiller O, Tschesche H, Consalvi V, Gallina C, Bioorg Med Chem Lett. 2006 Jan 1;16(1):20-4. Epub 2005 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16242329 16242329]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1zp5" style="background-color:#fffaf0;"></div>
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[[Category: Neutrophil collagenase]]
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[[Category: Single protein]]
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[[Category: Agamennone, M.]]
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[[Category: Biasone, A.]]
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[[Category: Campestre, C.]]
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[[Category: Gallina, C.]]
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[[Category: Gavuzzo, E.]]
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[[Category: Mazza, F.]]
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[[Category: Pochetti, G.]]
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[[Category: Preziuso, S.]]
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[[Category: Tortorella, P.]]
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[[Category: Tschesche, H.]]
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[[Category: 2NI]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:50 2008''
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==See Also==
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*[[Matrix metalloproteinase 3D structures|Matrix metalloproteinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Agamennone M]]
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[[Category: Biasone A]]
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[[Category: Campestre C]]
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[[Category: Gallina C]]
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[[Category: Gavuzzo E]]
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[[Category: Mazza F]]
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[[Category: Pochetti G]]
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[[Category: Preziuso S]]
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[[Category: Tortorella P]]
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[[Category: Tschesche H]]

Current revision

Crystal structure of the complex between MMP-8 and a N-hydroxyurea inhibitor

PDB ID 1zp5

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