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2a6o

From Proteopedia

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Crystal Structure of the ISHp608 Transposase in Complex with Stem-loop DNA

Structural highlights

2a6o is a 4 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q933Z0_HELPX

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many bacteria harbor simple transposable elements termed insertion sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements are particularly interesting due to their proximity to genes encoding gastric epithelial invasion factors. Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases. We have solved the crystal structure of full-length transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA does not resemble any characterized transposase; rather, it is related to rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a conserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate between TnpA and DNA. TnpA is dimeric, contains two shared active sites, and binds two DNA stem loops representing the conserved inverted repeats near each end of ISHp608. The cocrystal structure with stem-loop DNA illustrates how this family of transposases specifically recognizes and pairs ends, necessary steps during transposition.

Active site sharing and subterminal hairpin recognition in a new class of DNA transposases.,Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F Mol Cell. 2005 Oct 7;20(1):143-54. PMID:16209952^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F. Active site sharing and subterminal hairpin recognition in a new class of DNA transposases. Mol Cell. 2005 Oct 7;20(1):143-54. PMID:16209952 doi:10.1016/j.molcel.2005.07.026

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a6o"

@@ Line 3: / Line 3: @@
 <StructureSection load='2a6o' size='340' side='right'caption='[[2a6o]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2a6o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43504 Atcc 43504]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A6O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2a6o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6O FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a6m|2a6m]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6o OCA], [http://pdbe.org/2a6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a6o RCSB], [http://www.ebi.ac.uk/pdbsum/2a6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6o ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6o OCA], [https://pdbe.org/2a6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6o RCSB], [https://www.ebi.ac.uk/pdbsum/2a6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6o ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q933Z0_HELPX Q933Z0_HELPX]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 28: / Line 30: @@
 ==See Also==
-*[[Transposase|Transposase]]
+*[[Transposase 3D structures|Transposase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 43504]]
+[[Category: Helicobacter pylori]]
 [[Category: Large Structures]]
-[[Category: Chandler, M]]
+[[Category: Chandler M]]
-[[Category: Dyda, F]]
+[[Category: Dyda F]]
-[[Category: Ghirlando, R]]
+[[Category: Ghirlando R]]
-[[Category: Guynet, C]]
+[[Category: Guynet C]]
-[[Category: Perez, Z N]]
+[[Category: Perez ZN]]
-[[Category: Ronning, D R]]
+[[Category: Ronning DR]]
-[[Category: Ton-Hoang, B]]
+[[Category: Ton-Hoang B]]
-[[Category: Dna stem-loop]]
-[[Category: Rna recognition motif]]
-[[Category: Transcription-dna complex]]

2a6o

From Proteopedia

Current revision

Crystal Structure of the ISHp608 Transposase in Complex with Stem-loop DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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