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2bdz

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(New page: 200px<br /><applet load="2bdz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bdz, resolution 2.100&Aring;" /> '''Mexicain from Jacar...)
Current revision (07:37, 23 August 2023) (edit) (undo)
 
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[[Image:2bdz.jpg|left|200px]]<br /><applet load="2bdz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bdz, resolution 2.100&Aring;" />
 
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'''Mexicain from Jacaratia mexicana'''<br />
 
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==Overview==
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==Mexicain from Jacaratia mexicana==
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Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical, plant Jacaratia mexicana. Extracted as a mix of proteases from the latex, of the fruit, mexicain is isolated after cation-exchange chromatography as, the most abundant product. The purified product inhibited with E-64 was, crystallized by sitting-drop vapour diffusion in the presence of, ethanolamine. Cryoprotected crystals diffracted X-rays from a home source, to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell, parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The, asymmetric unit contains four molecules of mexicain, with a corresponding, crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent, content of 45% by volume. A molecular-replacement model has been, determined and refinement is in progress.
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<StructureSection load='2bdz' size='340' side='right'caption='[[2bdz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bdz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BDZ FirstGlance]. <br>
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2BDZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana] with <scene name='pdbligand=E64:'>E64</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bdz OCA], [https://pdbe.org/2bdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bdz RCSB], [https://www.ebi.ac.uk/pdbsum/2bdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bdz ProSAT]</span></td></tr>
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Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502326 15502326]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MEX1_JACME MEX1_JACME] Cysteine protease.[REFERENCE:1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/2bdz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bdz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Jacaratia mexicana]]
[[Category: Jacaratia mexicana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garcia-Ruiz, J.M.]]
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[[Category: Garcia-Ruiz JM]]
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[[Category: Gavira, J.A.]]
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[[Category: Gavira JA]]
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[[Category: Gonzalez-Ramirez, L.A.]]
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[[Category: Gonzalez-Ramirez LA]]
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[[Category: Oliver-Salvador, M.C.]]
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[[Category: Oliver-Salvador MC]]
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[[Category: Soriano-Garcia, M.]]
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[[Category: Soriano-Garcia M]]
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[[Category: E64]]
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[[Category: cysteine protease]]
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[[Category: mexicain]]
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[[Category: papain-like]]
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[[Category: peptidase_c1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:22:29 2008''
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Current revision

Mexicain from Jacaratia mexicana

PDB ID 2bdz

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