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2xpi

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[[Image:2xpi.jpg|left|200px]]
 
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==Crystal structure of APC/C hetero-tetramer Cut9-Hcn1==
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The line below this paragraph, containing "STRUCTURE_2xpi", creates the "Structure Box" on the page.
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<StructureSection load='2xpi' size='340' side='right'caption='[[2xpi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xpi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XPI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AUC:GOLD+(I)+CYANIDE+ION'>AUC</scene></td></tr>
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{{STRUCTURE_2xpi| PDB=2xpi | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xpi OCA], [https://pdbe.org/2xpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xpi RCSB], [https://www.ebi.ac.uk/pdbsum/2xpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xpi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CUT9_SCHPO CUT9_SCHPO]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase responsible for controlling cell cycle transitions, is a multisubunit complex assembled from 13 different proteins. Numerous APC/C subunits incorporate multiple copies of the tetratricopeptide repeat (TPR). Here, we report the crystal structure of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units. A C-terminal block of TPR motifs interacts with Hcn1, whereas an N-terminal TPR block mediates Cdc16/Cut9 self-association through a homotypic interface. This dimer interface is structurally related to the N-terminal dimerization domain of Cdc27, demonstrating that both Cdc16/Cut9 and Cdc27 form homo-dimers through a conserved mechanism. The acetylated N-terminal Met residue of Hcn1 is enclosed within a chamber created from the Cut9 TPR superhelix. Thus, in complex with Cdc16/Cut9, the N-acetyl-Met residue of Hcn1, a putative degron for the Doa10 E3 ubiquitin ligase, is inaccessible for Doa10 recognition, protecting Hcn1/Cdc26 from ubiquitin-dependent degradation. This finding may provide a structural explanation for a mechanism to control the stoichiometry of proteins participating in multisubunit complexes.
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===CRYSTAL STRUCTURE OF APC/C HETERO-TETRAMER CUT9-HCN1===
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The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27.,Zhang Z, Kulkarni K, Hanrahan SJ, Thompson AJ, Barford D EMBO J. 2010 Nov 3;29(21):3733-44. Epub 2010 Oct 5. PMID:20924356<ref>PMID:20924356</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xpi" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20924356 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20924356}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2xpi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XPI OCA].
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==Reference==
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<ref group="xtra">PMID:20924356</ref><references group="xtra"/>
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Barford, D.]]
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[[Category: Barford D]]
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[[Category: Kulkarni, K A.]]
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[[Category: Kulkarni KA]]
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[[Category: Zhang, Z.]]
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[[Category: Zhang Z]]

Current revision

Crystal structure of APC/C hetero-tetramer Cut9-Hcn1

PDB ID 2xpi

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