2yii

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[[Image:2yii.png|left|200px]]
 
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==Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution==
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The line below this paragraph, containing "STRUCTURE_2yii", creates the "Structure Box" on the page.
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<StructureSection load='2yii' size='340' side='right'caption='[[2yii]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2yii]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Taxus_chinensis Taxus chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YII FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>
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{{STRUCTURE_2yii| PDB=2yii | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yii OCA], [https://pdbe.org/2yii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yii RCSB], [https://www.ebi.ac.uk/pdbsum/2yii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yii ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAM_TAXWC PAM_TAXWC] Phenylalanine aminomutase that catalyzes the rearrangement of L-phenylalanine to R-beta-phenylalanine. Catalyzes the first commited step in the biosynthesis of the side chain of the alkaloid taxol (paclitaxel), a widely-used compound with antitumor activity. Has also low phenylalanine ammonia-lyase activity and can catalyze the amination of trans-cinnamate.<ref>PMID:15878763</ref> <ref>PMID:22113970</ref> <ref>PMID:24786474</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regioselective amination of cinnamate derivatives (see scheme, red) to, for example, beta-amino acids. This regioselectivity, along with the X-ray crystal structures, suggests two distinct carboxylate binding modes differentiated by C(beta)-C(ipso) bond rotation, which determines if beta- (see scheme) or alpha-addition takes place.
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===Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution===
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Mechanism-inspired engineering of phenylalanine aminomutase for enhanced beta-regioselective asymmetric amination of cinnamates.,Wu B, Szymanski W, Wybenga GG, Heberling MM, Bartsch S, de Wildeman S, Poelarends GJ, Feringa BL, Dijkstra BW, Janssen DB Angew Chem Int Ed Engl. 2012 Jan 9;51(2):482-6. doi: 10.1002/anie.201106372. Epub, 2011 Nov 23. PMID:22113970<ref>PMID:22113970</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2yii" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2yii]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Taxus_wallichiana_var._chinensis Taxus wallichiana var. chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YII OCA].
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*[[Aminomutase 3D structures|Aminomutase 3D structures]]
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[[Category: Taxus wallichiana var. chinensis]]
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== References ==
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[[Category: Bartsch, S.]]
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<references/>
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[[Category: Dijkstra, B W.]]
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__TOC__
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[[Category: Feringa, B L.]]
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</StructureSection>
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[[Category: Heberling, M M.]]
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[[Category: Large Structures]]
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[[Category: Janssen, D B.]]
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[[Category: Taxus chinensis]]
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[[Category: Poelarends, G J.]]
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[[Category: Bartsch S]]
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[[Category: Szymanski, W.]]
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[[Category: Dijkstra BW]]
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[[Category: Wildeman, S.]]
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[[Category: Feringa BL]]
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[[Category: Wu, B.]]
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[[Category: Heberling MM]]
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[[Category: Wybenga, G G.]]
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[[Category: Janssen DB]]
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[[Category: Lyase]]
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[[Category: Poelarends GJ]]
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[[Category: Szymanski W]]
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[[Category: Wildeman S]]
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[[Category: Wu B]]
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[[Category: Wybenga GG]]

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Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution

PDB ID 2yii

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