2g33

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{{Seed}}
 
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[[Image:2g33.png|left|200px]]
 
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==Human Hepatitis B Virus T=4 capsid, strain adyw==
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The line below this paragraph, containing "STRUCTURE_2g33", creates the "Structure Box" on the page.
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<StructureSection load='2g33' size='340' side='right'caption='[[2g33]], [[Resolution|resolution]] 3.96&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2g33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_B_virus_subtype_adyw Hepatitis B virus subtype adyw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G33 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.96&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g33 OCA], [https://pdbe.org/2g33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g33 RCSB], [https://www.ebi.ac.uk/pdbsum/2g33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g33 ProSAT]</span></td></tr>
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{{STRUCTURE_2g33| PDB=2g33 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_HBVD1 CAPSD_HBVD1] Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions (By similarity).<ref>PMID:7711014</ref> Encapsidates hepatitis delta genome (By similarity).<ref>PMID:7711014</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/2g33_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g33 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hepatitis B virus (HBV) is a leading cause of liver disease and hepatocellular carcinoma; over 400 million people are chronically infected with HBV. Specific anti-HBV treatments, like most antivirals, target enzymes that are similar to host proteins. Virus capsid protein has no human homolog, making its assembly a promising but undeveloped therapeutic target. HAP1 [methyl 4-(2-chloro-4-fluorophenyl)-6-methyl-2-(pyridin-2-yl)-1,4-dihydropyrimidin e-5-carboxylate], a heteroaryldihydropyrimidine, is a potent HBV capsid assembly activator and misdirector. Knowledge of the structural basis for this activity would directly benefit the development of capsid-targeting therapeutic strategies. This report details the crystal structures of icosahedral HBV capsids with and without HAP1. We show that HAP1 leads to global structural changes by movements of subunits as connected rigid bodies. The observed movements cause the fivefold vertices to protrude from the liganded capsid, the threefold vertices to open, and the quasi-sixfold vertices to flatten, explaining the effects of HAP1 on assembled capsids and on the assembly process. We have identified a likely HAP1-binding site that bridges elements of secondary structure within a capsid-bound monomer, offering explanation for assembly activation. This site also interferes with interactions between capsid proteins, leading to quaternary changes and presumably assembly misdirection. These results demonstrate the plasticity of HBV capsids and the molecular basis for a tenable antiviral strategy.
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===Human Hepatitis B Virus T=4 capsid, strain adyw===
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Global structural changes in hepatitis B virus capsids induced by the assembly effector HAP1.,Bourne CR, Finn MG, Zlotnick A J Virol. 2006 Nov;80(22):11055-61. Epub 2006 Aug 30. PMID:16943288<ref>PMID:16943288</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16943288}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2g33" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16943288 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16943288}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Hepatitis B virus subtype adyw]]
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2G33 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_b_virus_subtype_adyw Hepatitis b virus subtype adyw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G33 OCA].
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[[Category: Large Structures]]
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[[Category: Bourne CR]]
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==Reference==
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[[Category: Zlotnick A]]
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Global structural changes in hepatitis B virus capsids induced by the assembly effector HAP1., Bourne CR, Finn MG, Zlotnick A, J Virol. 2006 Nov;80(22):11055-61. Epub 2006 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16943288 16943288]
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[[Category: Hepatitis b virus subtype adyw]]
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[[Category: Single protein]]
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[[Category: Bourne, C R.]]
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[[Category: Zlotnick, A.]]
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[[Category: Capsid]]
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[[Category: Four-helix bundle]]
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[[Category: Hepadnavirus]]
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[[Category: Icosohedral]]
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[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:00:23 2008''
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Current revision

Human Hepatitis B Virus T=4 capsid, strain adyw

PDB ID 2g33

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