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2gk0

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[[Image:2gk0.jpg|left|200px]]<br /><applet load="2gk0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gk0, resolution 2.45&Aring;" />
 
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'''Structure of Catalytic Elimination Antibody 13G5 from a twinned crystal in space group C2'''<br />
 
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==Overview==
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==Structure of Catalytic Elimination Antibody 13G5 from a twinned crystal in space group C2==
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The structure of antibody 13G5, which catalyzes the conversion of unactivated benzisoxazoles to the corresponding salicylonitriles, was determined at 2.65 &Aring; resolution in the absence of ligand. It shows that the 2-aminobenzimidazolium derivative originally used for immunization was successful in templating an active site containing multiple functional groups. Thus, the side chains of three polar residues, AspH35, HisH95, and GluL34, project into an otherwise hydrophobic cavity. Site-directed mutagenesis and subsequent kinetic analysis identified AspH35 as the likely catalytic base that initiates proton abstraction. HisH95 appears to activate the base, whereas GluL34 may stabilize the incipient phenolate anion in the transition state at acidic pH. Introduction of alanine at position L34 unexpectedly boosts the activity of the catalyst substantially and broadens its pH optimum, perhaps because ordered water molecules are able to assume the role of proton donor over a wider range of conditions than the original glutamic acid. The aspartate base in the GluL34Ala variant provides an astonishing 109-fold rate advantage over the nonenzymatic reaction with acetate as base and exhibits an effective molarity of &gt;106 M. These large effects illustrate the utility of bifunctional catalysis in an antibody active site for promoting reactions with unactivated substrates.
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<StructureSection load='2gk0' size='340' side='right'caption='[[2gk0]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GK0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gk0 OCA], [https://pdbe.org/2gk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2gk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gk0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q65ZC0_MOUSE Q65ZC0_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/2gk0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gk0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GK0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GK0 OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Bifunctional catalysis of proton transfer at an antibody active site., Muller R, Debler EW, Steinmann M, Seebeck FP, Wilson IA, Hilvert D, J Am Chem Soc. 2007 Jan 24;129(3):460-1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17226987 17226987]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Debler EW]]
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[[Category: Debler, E.W.]]
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[[Category: Wilson IA]]
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[[Category: Wilson, I.A.]]
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[[Category: catalytic antibody]]
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[[Category: elimination]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:28:13 2008''
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Current revision

Structure of Catalytic Elimination Antibody 13G5 from a twinned crystal in space group C2

PDB ID 2gk0

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