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2gty

From Proteopedia

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Crystal structure of unliganded griffithsin

Structural highlights

2gty is a 2 chain structure with sequence from Griffithsia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.3Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GRFIN_GRISQ

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of griffithsin, an antiviral lectin from the red alga Griffithsia sp., was solved and refined at 1.3 A resolution for the free protein and 0.94 A for a complex with mannose. Griffithsin molecules form a domain-swapped dimer, in which two beta strands of one molecule complete a beta prism consisting of three four-stranded sheets, with an approximate 3-fold axis, of another molecule. The structure of each monomer bears close resemblance to jacalin-related lectins, but its dimeric structure is unique. The structures of complexes of griffithsin with mannose and N-acetylglucosamine defined the locations of three almost identical carbohydrate binding sites on each monomer. We have also shown that griffithsin is a potent inhibitor of the coronavirus responsible for severe acute respiratory syndrome (SARS). Antiviral potency of griffithsin is likely due to the presence of multiple, similar sugar binding sites that provide redundant attachment points for complex carbohydrate molecules present on viral envelopes.

Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding.,Ziolkowska NE, O'Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A Structure. 2006 Jul;14(7):1127-35. PMID:16843894^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ziolkowska NE, O'Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A. Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding. Structure. 2006 Jul;14(7):1127-35. PMID:16843894 doi:10.1016/j.str.2006.05.017

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gty"

Categories: Griffithsia | Large Structures | Wlodawer A | Ziolkowska NE

@@ Line 3: / Line 3: @@
 <StructureSection load='2gty' size='340' side='right'caption='[[2gty]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gty]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GTY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gty]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Griffithsia Griffithsia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GTY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2guc|2guc]], [[2gud|2gud]], [[2gue|2gue]], [[2gux|2gux]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gty OCA], [https://pdbe.org/2gty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gty RCSB], [https://www.ebi.ac.uk/pdbsum/2gty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gty ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gty OCA], [http://pdbe.org/2gty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gty RCSB], [http://www.ebi.ac.uk/pdbsum/2gty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gty ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GRFIN_GRISQ GRFIN_GRISQ]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 35: @@
 [[Category: Griffithsia]]
 [[Category: Large Structures]]
-[[Category: Wlodawer, A]]
+[[Category: Wlodawer A]]
-[[Category: Ziolkowska, N E]]
+[[Category: Ziolkowska NE]]
-[[Category: Domain swapping]]
-[[Category: Griffithsin]]
-[[Category: Hiv]]
-[[Category: Lectin]]
-[[Category: Mannose binding]]
-[[Category: Sar]]
-[[Category: Sugar binding protein]]

2gty

From Proteopedia

Current revision

Crystal structure of unliganded griffithsin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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