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2gyd

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[[Image:2gyd.gif|left|200px]]
 
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{{Structure
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==Complex of equine apoferritin with the H-diaziflurane photolabeling reagent==
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|PDB= 2gyd |SIZE=350|CAPTION= <scene name='initialview01'>2gyd</scene>, resolution 1.720&Aring;
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<StructureSection load='2gyd' size='340' side='right'caption='[[2gyd]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> and <scene name='pdbligand=DFE:2-(1,1-DIFLUOROETHOXY)-1,1,1-TRIFLUOROETHANE'>DFE</scene>
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<table><tr><td colspan='2'>[[2gyd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GYD FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=DFE:2-(1,1-DIFLUOROETHOXY)-1,1,1-TRIFLUOROETHANE'>DFE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gyd OCA], [https://pdbe.org/2gyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gyd RCSB], [https://www.ebi.ac.uk/pdbsum/2gyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gyd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FRIL_HORSE FRIL_HORSE] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gy/2gyd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gyd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.
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'''Complex of equine apoferritin with the H-diaziflurane photolabeling reagent'''
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Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions.,Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:17163775<ref>PMID:17163775</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2gyd" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2GYD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYD OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17163775 17163775]
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[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Eckenhoff, R.]]
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[[Category: Eckenhoff R]]
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[[Category: Loll, P J.]]
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[[Category: Loll PJ]]
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[[Category: Rossi, M J.]]
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[[Category: Rossi MJ]]
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[[Category: CD]]
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[[Category: DFE]]
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[[Category: anesthetic]]
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[[Category: helical bundle]]
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[[Category: isoflurane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:10:24 2008''
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Current revision

Complex of equine apoferritin with the H-diaziflurane photolabeling reagent

PDB ID 2gyd

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