2h1g

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(New page: 200px<br /><applet load="2h1g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h1g, resolution 3.100&Aring;" /> '''ResA C74A/C77A'''<b...)
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[[Image:2h1g.gif|left|200px]]<br /><applet load="2h1g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h1g, resolution 3.100&Aring;" />
 
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'''ResA C74A/C77A'''<br />
 
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==Overview==
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==ResA C74A/C77A==
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ResA, an extracytoplasmic thioredoxin from Bacillus subtilis, acts in, cytochrome c maturation by reducing the disulfide bond present in, apocytochromes prior to covalent attachment of heme. This reaction is (and, has to be) specific, as broad substrate specificity would result in, unproductive shortcircuiting with the general oxidizing thioredoxin(s), present in the same compartment. Using mutational analysis and subsequent, biochemical and structural characterization of active site variants, we, show that reduced ResA displays unusually low reactivity at neutral pH, consistent with the observed high pKa values&gt;8 for both active site, cysteines. Residue Glu80 is shown to play a key role in controlling the, acid-base properties of the active site. A model in which substrate, binding dramatically enhances the reactivity of the active site cysteines, is proposed to account for the specificity of the protein. Such a, substratemediated activation mechanism is likely to have wide relevance, for extracytoplasmic thioredoxins.
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<StructureSection load='2h1g' size='340' side='right'caption='[[2h1g]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2h1g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H1G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1g OCA], [https://pdbe.org/2h1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h1g RCSB], [https://www.ebi.ac.uk/pdbsum/2h1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h1g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RESA_BACSU RESA_BACSU] Thiol-disulfide oxidoreductase which is required in disulfide reduction during c-type cytochrome synthesis. May accept reducing equivalents from CcdA, leading to breakage of disulfide bonds in apocytochrome c; following this reduction heme can be covalently attached. Does not play a role in sporulation.<ref>PMID:12637552</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h1/2h1g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h1g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ResA, an extracytoplasmic thioredoxin from Bacillus subtilis, acts in cytochrome c maturation by reducing the disulfide bond present in apocytochromes prior to covalent attachment of heme. This reaction is (and has to be) specific, as broad substrate specificity would result in unproductive shortcircuiting with the general oxidizing thioredoxin(s) present in the same compartment. Using mutational analysis and subsequent biochemical and structural characterization of active site variants, we show that reduced ResA displays unusually low reactivity at neutral pH, consistent with the observed high pKa values&gt;8 for both active site cysteines. Residue Glu80 is shown to play a key role in controlling the acid-base properties of the active site. A model in which substrate binding dramatically enhances the reactivity of the active site cysteines is proposed to account for the specificity of the protein. Such a substratemediated activation mechanism is likely to have wide relevance for extracytoplasmic thioredoxins.
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==About this Structure==
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Molecular basis for specificity of the extracytoplasmic thioredoxin ResA.,Lewin A, Crow A, Oubrie A, Le Brun NE J Biol Chem. 2006 Nov 17;281(46):35467-77. Epub 2006 Sep 13. PMID:16971393<ref>PMID:16971393</ref>
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2H1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2H1G OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecular basis for specificity of the extracytoplasmic thioredoxin ResA., Lewin A, Crow A, Oubrie A, Le Brun NE, J Biol Chem. 2006 Nov 17;281(46):35467-77. Epub 2006 Sep 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16971393 16971393]
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</div>
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[[Category: Bacillus subtilis]]
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<div class="pdbe-citations 2h1g" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Brun, N.E.Le.]]
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[[Category: Crow, A.]]
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[[Category: Lewin, A.]]
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[[Category: Oubrie, A.]]
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[[Category: c74a]]
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[[Category: c77a]]
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[[Category: double mutant]]
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[[Category: resa]]
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[[Category: resa c74a/c77a]]
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[[Category: thioredoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:30:04 2007''
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==See Also==
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*[[Protein disulfide oxidoreductase|Protein disulfide oxidoreductase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus subtilis]]
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[[Category: Large Structures]]
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[[Category: Crow A]]
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[[Category: Le Brun NE]]
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[[Category: Lewin A]]
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[[Category: Oubrie A]]

Current revision

ResA C74A/C77A

PDB ID 2h1g

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